Fig. 6

The cell wall invertase activity and hexose content were induced by chilling stress and regulated by INVINH1. a Expression of INVINH1 in the first mature leaves of INVINH1 over-expression, wildtype and RNAi plants before and after treated at 4 °C for 24 h. Actin was used as the loading control. b Expression of cell wall invertase gene Lin6 in the first mature leaves of INVINH1 over-expression, wildtype and RNAi plants before and after treated at 4 °C for 24 h. Actin was used as the loading control. c The activity of cell wall invertase in the first mature leaves of INVINH1 over-expression and RNAi plants before and after treated at 4 °C for 24 h. d Sugar levels in the first mature leaves of INVINH1 over-expression, wildtype and RNAi plants before and after treated at 4 °C for 24 h. Each value in (a), (b), (c) and (d) is mean ± SE of at least four biological replicates. Lowercase letters indicate values significantly different at P < 0.05