Fig. 9

Regulation of NCED1 gene expression in INVINH1 over-expression and RNAi plants. a NCED1 expression in INVINH1 over-expression and RNAi plants before and after treated at 4 °C for 24 h. Actin was used as the loading control. b NCED1 expression in INVINH1 over-expression and RNAi plants before and after cold stress. The plants were stress-treated at 4 °C for 24 h and then recovered at 25 °C for 24H. Actin was used as the loading control. c Endogenous ABA levels in the first mature leaves of INVINH1 over-expression, wildtype and RNAi plants before and after treated at 4 °C for 24 h. d The shoots from the same position of 60-d INVINH1 RNAi, INVINH1 over expression and wildtype plant were immediately cut under water then treated at 4 °C of 24 h. e The shoots from the same position of 60-d INVINH1 RNAi, INVINH1 over expression and wildtype plant were immediately cut under water with 10 μM ABA then treated at 4 °C of 24 h. Each value in (a), (b) and (c) is mean ± SE of at least four biological replicates. Lowercase letters indicate values significantly different at P < 0.05