Fig. 2From: An optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transportProtein composition of the purified thylakoid membranes determined by (a) blue-native (BN)-PAGE (left lane: unstained sample, right lane: coomassie stained) and (b) SDS-PAGE. For the BN-PAGE thylakoids were solubilised with a Chl concentration of 1 μg μL−1 and 8 μL were applied to the gel, for SDS-PAGE thylakoids were applied with a total Chl content of 1.5 μg to the gel. For additional information see the Methods section. PSI: photosystem I, PSII: photosystem II (d: dimeric state, m: monomeric state), FCP: fucoxanthin-chlorophyll binding protein, MW: molecular weight markersBack to article page