Fig. 3

Transcriptional activity assay of the full-length and truncated heat-responsive TaMYB proteins in yeast. The length of each TaMYB protein and respective protein truncation are indicated. Fusion proteins of the GAL4 DNA-binding domain and full-length TaMYBs or truncated TaMYBs with deletion of X C-terminal amino acid residues (CΔX) were expressed in yeast strain AH109. The empty pGBKT7 vector and the pGBKT7-P53 construct were used as negative and positive controls, respectively. Equal amounts of liquid cultures of the transformed yeast cells were dropped onto SD plates containing no tryptophan (Trp) and onto SD plates without Trp and histidine (His)