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Fig. 1 | BMC Plant Biology

Fig. 1

From: The miR172c-NNC1 module modulates root plastic development in response to salt in soybean

Fig. 1

Analysis of the miR172c promoter and miR172c expression. a Computational analysis of the regulatory cis elements of the miR172c promoter. The promoter sequence (2000 bp) of upstream of pre-miR172c (www.phytozome.net/) was chosen for multiple cis-element analysis using an online software at http://www.dna.affrc.go.jp/place/. b qRT-PCR analysis of expression of miR172c in roots. Seven day-old seedlings were treated with 75 mM NaCl, and roots were harvested at 0, 1, 3, 6, 12, and 24 h after treatment. miR1515a was used to normalize transcript abundance. Data are mean ± SD from three biological repeats. Letters indicate significant differences from the empty vector controls according to the Student’s Newman-Kuels test (P < 0.05). c Histochemical analysis of the expression of miR172c. GUS staining was performed using the transgenic roots expression promiR172c::GUS treated with or without 75 mM NaCl for 24 h. Two weeks-old composite plants were used to conduct the experiments. Bars in (c) =1 cm

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