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Table 2 Full set of fruit proteins differentially abundant in (A) cv. Aragon, (B) cv. Gladis

From: Target proteins reprogrammed by As and As + Si treatments in Solanum lycopersicum L. fruit

aspot n°

bProtein name

cGene name

dOrganism

eScore

fCov. %

gExp. Mr. (kDa)/pI

hTh. Mr. (kDa)/pI

iBLAST protein

l gene name

midentity %

nTh. Mr. (kDa)

oCov. %

A)

202

UPF0725 protein At1g27860

At1g27860

A. thaliana

31

22

31/4,5

33,4/5,41

uncharacterized protein LOC101252956 (predicted)

Solyc03g005820.2

29

25,5

34

1204

14-3-3 protein 2

Solyc12g057110.2

S. lycopersicum

32

22

30/4,5

29,0/4,72

     

2103

calmodulin-like protein 3

CML3

O. sativa

24

44

23/5,0

20,3/4,62

calmodulin

Solyc03g098050.2

65

16,8

84

3405

actin-51 (Fragment)

Solyc11g005330.1

S. Lycopersicum

110

41

40/5,5

37,3/5,28

     

3602

probable lipid-A-disaccharide synthase, mitochondrial

LPXB

A. thaliana

27

7

55/5,5

51,9/9,0

probable lipid-A-disaccharide synthase, mitochondrial (predicted)

Solyc07g049740.2

60

51,8

94

4302

annexin p34

Solyc04g073990.2

S. Lycopersicum

100

88

35/5,5

35,7/6,41

     

4803

ATP-dependent Clp protease ATP-binding subunit clpA homolog CD4B, chloroplastic

Solyc12g042060.1

S. Lycopersicum

28

28

>97/5,5

102,5/5,8

     

5004

pathogenesis-related protein STH-2-like

Solyc09g090980.2

S. Lycopersicum

78

98

18/5,5

17,2/5,5

     

5305

cyclin-D6-1

CYCD6-1

T. aestivum

42

12

35/5,8

34,6/5,5

putative cyclin-D6-1

Solyc07g054950.1

33

35,4

80

5602

seed biotin-containing protein SBP65

SBP65

P. sativum

75

42

60/5,8

59,6/5,9

seed biotin-containing protein SBP65-like isoform X1

Solyc10g008040.2

34

66,1

62

5702

flavonol sulfotransferase-like

N/A

F. bidentis

68

49

66/6,0

36,2/5,8

cytosolic sulfotransferase 12-like (predicted)

Solyc05g011870.1

45

38,6

98

5706

nucleolin 2

NUCL2

A. thaliana

41

22

70/6,2

69,0/5,0

nucleolin 1 isoform X1 (predicted)

Solyc02g021220.1

47

65,7

53

6101

eukaryotic translation initiation factor 5A-2

Solyc07g005560.2.1

S. Lycopersicum

89

76

20/6,5

17,7/5,8

     

6102

small heat shock protein, chloroplastic

Solyc03g082420.2

S. Lycopersicum

67

56

25/6,3

26,3/7,8

     

6103

PHD finger protein ALFIN-LIKE 3

OsI_14081

O. sativa

50

44

25/6,5

28,3/5,7

PHD finger protein ALFIN-LIKE 2-like (predicted)

Solyc10g076690.1

70

27

93

6303

ADP, ATP carrier protein 2, mitochondrial

ANT-G2

T. aestivum

47

48

35/6,2

35,9/9,0

ADP/ATP translocator

Solyc11g062130.1

88

41,9

98

7103

protein LURP-one-related 1

At1g33840

A. thaliana

41

37

23/6,6

25,7/8,6

protein LURP-one-related 10-like (predicted)

Solyc10g085420.1

53

23,8

82

7503

alcohol dehydrogenase 2

ADH2

S. Lycopersicum

49

49

46/6,8

41,8/6,0

     

7705

F-box/WD-40 repeat-containing protein At5g21040

At5g21040

A. thaliana

40

33

66/6,7

61,1/8,5

F-box/WD-40 repeat-containing protein At5g21040-like (predicted)

Solyc06g073650.1

59

60,9

100

8105

growth-regulating factor 10

GRF10

O. sativa

43

16

22/6,8

22,5/9,0

growth-regulating factor 9-like (predicted)

Solyc08g079800.2

59

24,6

51

8202

VQ motif-containing protein 4

VQ4

A. thaliana

39

32

28/6,7

26,96/9,8

uncharacterized protein LOC101257285 (predicted)

Solyc02g078030.1

62

25,1

79

8701

V-type proton ATPase catalytic subunit

N/A

B. vulgaris

40

37

66/6,8

68,8/5,1

vacuolar H + −ATPase A2 subunit isoform

Solyc06g063330.2

92

68,5

100

B)

302

E3 ubiquitin-protein ligase CHIP

CHIP

A. thaliana

27

42

35/4,7

32,1/6,1

E3 ubiquitin-protein ligase CHIP (predicted)

Solyc06g083150.2

69

31,6

97

502

F-box protein At4g00755

At4g00755

A. thaliana

24

22

44/4,4

43,1/4,9

F-box protein At4g00755-like (predicted)

Solyc03g026170.2

54

41,1

81

1003

Heavy metal-associated isoprenylated plant protein

HIPP26

A. thaliana

26

35

15/5,0

17,2/9,3

heavy metal-associated isoprenylated plant protein 26-like (predicted)

Solyc01g111600.2

85

17,2

100

1305

GDSL esterase/lipase EXL4

EXL4

A. thaliana

18

17

40/5,1

38,3/9,0

GDSL esterase/lipase EXL3-like (predicted)

Solyc04g082390.2

42

43,7

91

1401

SKP1-like protein 21

ASK21

A. thaliana

18

18

40/5,0

40,3/5,6

SKP1-like protein 21 isoform X1 (predicted)

Solyc06g036070.2

65

40,1

96

1803

heat shock 70 kDa protein

HSP70

Z. mays

36

26

70/5,2

70,8/5,22

heat shock cognate 70 kDa protein 1

Solyc10g086410.2

89

70,9

99

2802

cell division cycle protein 48 homolog

CDC48

G. max

69

33

97/5,3

90,5/5,2

cell division cycle protein 48 homolog (predicted)

Solyc06g074980.2

93

90,1

100

2805

sucrose synthase 7

SUS7

O. sativa

25

9

97/5,3

98,4/8,0

sucrose synthase 7-like (predicted)

Solyc02g081300.2

69

96,2

98

3302

dihydroflavonol-4-reductase

Solyc02g085020.2.1

S. Lycopersicum

24

25

35/5,4

42,7/6,0

     

3401

1-aminocyclopropane-1-carboxylate oxidase homolog isoform 1

Solyc09g089580.2.1

S. Lycopersicum

71

38

40/5,5

40,9/5,6

     

3601

ATP synthase subunit beta, chloroplastic

Solyc01g007320.2.1

S. Lycopersicum

48

43

50/5,4

53,5/5,3

     

3801

subtilisin-like protease SBT1.7

SBT1.7

A. thaliana

26

15

97/5,5

80,1/5,9

SBT1 protein precursor

Solyc04g078110.1

64

78,6

97

4702

calcium-dependent protein kinase 18

CPK18

A. thaliana

48

22

64/5,6

60,4/8,7

calcium-dependent protein kinase

Solyc03g033540.2

80

63,7

89

5303

ADP,ATP carrier protein 2, mitochondrial

ANT-G2

T. aestivum

47

48

35/6,2

35,9/9,0

ADP/ATP translocator

Solyc11g062130.1

88

41,9

98

5601

enolase

Solyc09g009020.2.1

S. Lycopersicum

147

64

48/5,8

48,0/5,7

     

6502

alcohol dehydrogenase 2

Solyc06g059740.2.1

S. Lycopersicum

31

34

42/6,4

41,7/6,0

     

6606

CBL-interacting protein kinase 14

CIPK14

O. sativa

37

26

49/6,4

50,5/9,0

CBL-interacting protein kinase

Solyc05g052270.1

60

49,7

100

6607

26S protease regulatory subunit 7 homolog A

RPT1A

A. thaliana

45

25

50/6,4

48,2/6,3

26S protease regulatory subunit 7 homolog A-like (predicted)

Solyc06g063140.2

98

47,7

99

6701

phosphomethylpyrimidine synthase, chloroplastic

THIC

A. thaliana

69

30

70/6,4

72,6/6,0

phosphomethylpyrimidine synthase, chloroplastic (predicted)

Solyc06g006080.2

83

72,5

99

7203

SufE-like protein 2, chloroplastic

SUFE2

A. thaliana

26

23

28/6,5

29,1/9,0

sufE-like protein 2, chloroplastic isoform X2 (predicted)

Solyc05g013710.2

50

29,9

91

7603

cytochrome P450 85A1

CYP85A1

A. thaliana

28

34

55/6,5

54,1/9,1

cytochrome P450 85A1

Solyc02g089160.2

70

53,5

96

7605

mannan endo-1,4-beta-mannosidase 3

Solyc12g013750.1

S. Lycopersicum

34

24

48/6,5

45,3/5,6

     

7701

linoleate 9S-lipoxygenase B

Solyc01g099190.2.1

S. Lycopersicum

74

22

60/6,4

97,5/5,6

     

7702

argininosuccinate lyase, chloroplastic

At5g10920

A. thaliana

28

23

60/6,4

57,9/5,6

argininosuccinate lyase

Solyc04g076320.2

80

nd

89

7703

F-box/WD-40 repeat-containing protein At5g21040

At5g21040

A. thaliana

40

33

66/6,7

61,1/8,5

F-box/WD-40 repeat-containing protein At5g21040-like (predicted)

Solyc06g073650.1

59

60,9

100

7704

zinc finger CCCH domain-containing protein 46

At3g51950

A. thaliana

29

23

60/6,5

60,2/6,5

zinc finger CCCH domain-containing protein 46-like isoform X2 (predicted)

Solyc01g008600.2

46

61,9

79

7705

pentatricopeptide repeat-containing protein At1g15480, mitochondrial

At1g15480

A. thaliana

29

17

66/6,4

67,8/6,4

pentatricopeptide repeat-containing protein At1g80270, mitochondrial (predicted)

Solyc12g020050.1

77

70,1

59

8107

growth-regulating factor 10

GRF10

O. sativa

43

16

22/6,8

22,5/9,0

growth-regulating factor 9-like (predicted)

Solyc08g079800.2

59

24,6

51

8205

40S ribosomal protein S3a-2

RPS3AB

A. thaliana

29

35

28/6,7

29,9/9,7

40S ribosomal protein S3a (predicted)

Solyc06g072490.2

84

29,6

92

8304

aldo-keto reductase family 4 member C11

AKR4C11

A. thaliana

37

18

35/6,6

35,2/6,5

aldo-keto reductase family 4 member C9 (predicted)

Solyc00g015750.1

78

35,0

100

  1. Proteins abundance varied in response tothe As and/or As + Si treatments. Notes: a spot number assigned following 2DE separation, b putative protein identification, c encoding gene of the closest match in the Swiss-Prot database, d organism, e score, f percentage of coverage of the matching peptide sequence tags derived from MASCOT analysis, g estimated mass and pI, h predicted mass and pI of the closest match, i best BLAST hit in tomato, l gene name, m predicted mass, n % identity, o % coverage identified by BLASTp search of tomato