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Fig. 6 | BMC Plant Biology

Fig. 6

From: Mitochondrial pyruvate carrier 1 mediates abscisic acid-regulated stomatal closure and the drought response by affecting cellular pyruvate content in Arabidopsis thaliana

Fig. 6

Pyruvate promoted stomatal closure by activating slow-type anion channel activity. a The induction of stomatal closure by 10–1000 μM pyruvate. Values are shown as means ± SE (n = 3). Before width/length ratio analysis, at least 30 stomata were measured. Statistically significant (P < 0.05) differences are indicated by different letters. b Representative stomata in control and pyruvate-treated plants. Bar: 10 μm. c Patch clamp whole-cell recordings of slow-type anion currents in guard cell protoplasts treated with either 50 μM ABA or 100 μM pyruvate. d Current/voltage relationships of whole-cell slow-type anion currents, as illustrated in (c). The numbers of guard cells measured were as follows: Control, n = 15; ABA, n = 11; and Pyruvate, n = 14. Values are means ± SE

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