- Research article
- Open Access
Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane
BMC Plant Biology volume 17, Article number: 213 (2017)
In thylakoid membrane, each monomer of the dimeric complex of cytochrome b 6 f is comprised of eight subunits that are both nucleus- and plastid-encoded. Proper cytochrome b 6 f complex integration into the thylakoid membrane requires numerous regulatory factors for coordinated transport, insertion and assembly of the subunits. Although, the chloroplast-encoded cytochrome b 6 f subunit IV (PetD) consists of three transmembrane helices, the signal and the mechanism of protein integration into the thylakoid membrane have not been identified.
Here, we demonstrate that the native PetD subunit cannot incorporate into the thylakoid membranes spontaneously, but that proper integration occurs through the post-translational signal recognition particle (SRP) pathway. Furthermore, we show that PetD insertion into thylakoid membrane involves the coordinated action of cpFTSY, cpSRP54 and ALB3 insertase.
PetD subunit integration into the thylakoid membrane is a post-translational and an SRP-dependent process that requires the formation of the cpSRP-cpFtsY-ALB3-PetD complex. This data provides a new insight into the molecular mechanisms by which membrane proteins integration into the thylakoid membrane is accomplished and is not limited to PetD.
The 220-kDa multiprotein cytochrome b 6 f complex located in the thylakoid membrane provides the electronic connection between the photosystem I (PSI) and II (PSII) reaction centers in the electron transport chain of oxygenic photosynthesis [1, 2]. The cytochrome b 6 f complex is composed of four major subunits, cytochrome f (PetA), a Rieske-type iron–sulfur protein, cytochrome b 6 (PetB), and the chloroplast-encoded subunit IV (PetD), all of which are required for the full catalytic activity of the complex . Furthermore, up to four additional small subunits may also be present in isolated b 6 f complex preparations .
The chloroplast oligomeric complexes that form the photosynthetic electron transfer chain of the thylakoid membrane of higher plants (including cytochrome b 6 f, PSI, PSII and ATPase) consist of a patchwork of nuclear- and chloroplast-encoded components. Hence, integration of these complexes into the thylakoid membrane requires the assembly of subunits of both chloroplast and nuclear origin and relies on the coordinated action of numerous regulatory factors [5, 6]. However, the majority of plastid proteins are nuclear-encoded and must be imported within these organelles. Targeting of nuclear-encoded proteins to plastid compartments such as the inner envelope membrane, the stroma, and the thylakoid membrane is strictly dependent on the presence of a cleavable transit sequence in the precursor N-terminal region. However, the mechanisms underlying the biosynthesis as well as the targeting to plastid compartments of the chloroplast-encoded proteins, is less understood.
The chloroplast encoded proteins can be imported into or across the thylakoid membrane by one of four independent precursor-specific transport pathways that are categorized as the spontaneous (unassisted) pathway, the signal recognition particle (SRP) pathway, the secretory pathway (Sec) that requires protein substrates to be in an unfolded state for transport, and the twin-arginine translocase-dependent pathway (ΔpH/Tat) that protein substrates can be transported in a folded state, allowing the transport of proteins that fold too quickly or tightly for the Sec pathway. Interestingly, although the Tat pathway post-translationally transports folded proteins mainly across the cytoplasmic membrane, the Tat machinery mediates the insertion of Rieske iron-sulfur protein (a member of cytochrome b 6 f complex) into the thylakoid membrane in plants [7,8,9]. Hence, incorporation into the thylakoid membrane for some proteins occurs post-translationally, while others fold and integrate co-translationally such as PetB, PsaA, PsbB, PsbC, PsbD, and PetA [10,11,12]. The results, however, for PetA incorporation into the thylakoid membrane are inconsistent [12, 13].
During co-translational incorporation into membranes, polypeptides that are being synthesized on the membrane bound ribosomes (nascent polypeptides) are translocated or integrated by the translocase while the ribosomes remain bound to the translocation apparatus. The membrane integration of nascent polypeptides requires a cleavable signal sequence or a TMH that provides signal anchor . Post-translational incorporation takes place on cytosolic free ribosomes. Following their synthesis proteins are discharged into the cytosol, and some of these proteins last in quasi-soluble form. Whereas others containing an N-terminal hydrophobic signal sequence are identified by the signal recognition particle (SRP), and this facilitates their association with the receptor proteins and then their delivery to the pore-forming membrane protein translocation channel, where the proteins are directly integrated into the membrane. However, as in case of Light-Harvesting Chlorophyll a/b Binding Proteins (LHCPs) insertion into membrane, the post-translational SRP-dependent pathway may use an integral signal sequence as well . Normally, the conserved universal SRP pathway usually mediates both co-translation and post-translational targeting. However, a unique chloroplast SRP has also been discovered in green plants . This novel chloroplast pathway involves cpSRP54 and its membrane receptor cpFtsY, two GTPases that are similar to the cytosolic SRP54 and SR GTPases, and a unique 43-kDa protein, cpSRP43 [16,17,18]. Furthermore, a membrane-bound homologue of bacterial YidC, termed ALB3, is also involved in the SRP pathway . Importantly, the mechanism and signal for this integration within the thylakoid membrane of the chloroplast-encoded cytochrome b 6 f subunit IV (PetD) that consists of three transmembrane helices (TMH) (Fig. 1c)  remains unknown .
Previous analysis of thylakoid-bound ribosomes proposed a post-translational mechanism of PetD integration [12, 22]. The post-translationally integrating thylakoid proteins included the multi-spanning proteins PetD, AtpH, PsbK, and PsbZ that were proposed to integrate into membrane without the aid of a proteinaceous machinery . To date, no one has verified this mechanism of PetD integration and assembly into the thylakoid membrane. Therefore, the goal of the present study was to investigate the molecular mechanism of PetD integration into the membrane in the chloroplast environment.
Our comparative analysis revealed that although PetD interacts directly with the thylakoid membrane by hydrophobic interactions, it can easily be removed with chaotropic agents. These results exclude the spontaneous pathway for insertion of PetD into the thylakoid membrane, and indicate that other proteins mediate the proper integration of PetD post-translationally. We determined that PetD is inserted into thylakoid membrane by the coordinated action of cpFTSY, cpSRP54 and ALB3 insertase. Hence, we propose that the chloroplast SRP pathway is the mechanism responsible for the post-translational membrane assembly of the chloroplast-encoded cytochrome b 6 f subunit IV (PetD).
TargetP 1.1 server (www.cbs.dtu.dk/services/TargetP) was used to predict the presence of the chloroplast transit peptides (cTP) in the protein sequences and the location of potential cTP cleavage sites. TargetP has a substantially greater ability to discriminate between signal peptides and uncleaved signal anchors .
Kyte–Doolittle hydropathy plots  were generated using an online tool available at the ExPASy molecular biology server (https://web.expasy.org/protscale/). For in-silico multiple sequence alignments, the MAFFT version 7-alignment software (https://mafft.cbrc.jp/alignment/server/) was used. . PredSL (http://aias.biol.uoa.gr/PredSL/) was used to predict a protein’s localization . The PSORT Prediction tool (http://psort1.hgc.jp/form.html) that uses the overall amino acid composition, the N-terminal targeting sequence information, and the motifs, was used as a hybrid approach. This latter software uses a set of knowledge-based “if-then” rules .
Isolation of thylakoid membranes and stroma fractions from intact chloroplasts
Pea seeds (Pisum sativum, cv Calvedon) were grown hydroponically and intact chloroplast from leaves was isolated as described previously . Washed thylakoids and stromal extract was prepared from isolated chloroplasts as described previously . Total chlorophyll (CHL) content was measured as described previously . Prior to the experiments, reconstituted lysates were prepared by suspending the thylakoid membranes in the freshly prepared stroma.
Preparation and analysis of free and bound ribosomes
Cell free transcription-translation insertion assays
The recombinant plasmid pET25b-SUIV containing the gene PetD was used as a template for the creation of plasmid pT7CFE1-SUIV (Additional file 1: Figure S1). The pT7CFE1-CHis based expression construct, pT7CFE1-SUIV, was used to express the PetD gene. PetD was cloned between the MscI and BamHI restriction sites of the PT7CFE1-CHis vector (Thermo Fisher Scientific). To eliminate the need for affinity tags (His tag), a double stop codon was added at the 3′ end of the PetD sequence. The pT7CFE1-SUIV expression vector utilizes the T7 viral promoter and an encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) that is critical for high levels of cap-independent protein expression. Moreover, this expression vector possesses a T7 RNA polymerase promoter that functions with the T7 RNA polymerase that is included in the transcription reaction mixture. The obtained pT7CFE1-SUIV construct was confirmed to be correct by sequence analysis. The plasmid pT7CFE1-b 6 is described in .
Coupled transcription/translation reactions of the pea PetD were performed as described in  with the following modifications: the PetD gene was firstly transcribed using pT7CFE1-SUIV construct as a template and the 2.0 μg of DNA-purified mRNA was used in the translation process. PetD mRNA was then translated in vitro in the presence or absence of thylakoid membranes, with or without the freshly prepared stromal fraction for 60 min at 30 °C in the presence of 10 μCi [35S]-methionine (Hartmann Analytic, Germany). Following the translation reaction, another 2.0 μg of the DNA-purified mRNA and 1 μL of manufacture “energy mix” were added for each reaction and incubated for another 30 min in order to increase the protein yield. The translation in vitro assays that were required for the post-translational insertion of PetD were performed accordingly to the method of Houben, et al.  with the following modifications: after 60 min of translation in the presence of 10 μCi [35S]-methionine, puromycin and lincomycin were added to a final concentration of 5 mM and 15 mM, respectively, and the samples were incubated for another 30 min at 30 °C. Subsequently, membranes and protease inhibitors were added, and the samples were kept for 30 min at 30 °C. The membranes were separated from the translation mix and then purified as described previously . Finally, in order to remove the endogenous RNA as well as the external domains of the endogenous proteins the translation reactions, the thylakoid membranes or stroma were pre-incubated as described in .
Assessment of integration of PetD into the thylakoid membranes
Assay mixtures containing 45 μL thylakoid suspension (250 μg CHL /mL) in HM buffer and 25 μL of translation mixture were incubated for 20 min at 25 °C. Following double washing of the thylakoids suspensions, the samples were analyzed. Where appropriate, incubations were pretreated with 1 unit of apyrase or sodium azide (10 mM) or nigericin (2 μM) plus KCl (10 mM).
To monitor the integration of the targeted proteins, membranes (200 μg CHL /mL) were resuspended in 2 M NaBr or 2 M NaSCN in 50 mM HEPES-KOH pH 7.7 buffer containing a protease inhibitor cocktail  and incubated on ice for 30 min. Next, the membranes were washed and collected (20,000×g for 10 min). Although the resulting membranes were solubilized in SDS sample buffer directly (pellet fraction), the supernatant fraction was precipitated in 80% acetone and then analysed by SDS–PAGE. The proteolytic assessment of protein import into the thylakoid membranes was performed as described in  with modifications made by Kroliczewski, et al. .
Integration of the single span subunit W of PS II (PsbW)
In order to validate PetD insertion assays, we followed the thylakoid membrane integration of PsbW using the methods described in . PsbW protein inserts into the thylakoid membrane by the spontaneous pathway .
Crosslinking and co-immunoprecipitation of the ribosomal fractions
The ribosome-nascent chain complexes (RNCs) were cross-linked on ice using the homobifunctional N-hydroxysuccinade ester bis[2-(succinimidyloxycarbonyloxy) ethyl] sulfone (BSOCOES, Pierce), a membrane-permeable cleavable crosslinker or the heterobifunctional cleavable (by DTT) crosslinker N-succinimidyl-3-[2-pyridyldithio]propionate (SPDP, Pierce) by the method of Kroliczewski, et al.  followed by co-immunoprecipitation reactions using an antibody (Ab) against ALB3 or cpSRP54 proteins [11, 28]. To perform the immunoprecipitation reaction, we used 2 μg of antibody. The immunoprecipitated proteins were analysed by SDS-PAGE and Western blot or subjected to autoradiography using X-ray cassettes with a sheet of X-ray film (Kodak) . The gels were dried prior to being placed on X-ray film. Furthermore, the detected bands were excised from a polyacrylamide gel and analysed by Q-TOF MS/MS spectrometry (MS) with peptide mass fingerprints (PMF), (see: Mass spectroscopy analysis section).
PetD and PetB co-expression in cell-free assays
The cell-free expression assays were performed for 60 min to ensure synchronised expression of PetD and PetB  in the presence of stroma and membrane fractions followed by crosslinking with 25 mM BSOCOES or 40 mM SPDP. BSOCOES was added to the protein samples to achieve a concentration equal to 10–50 times the molar concentration of the proteins in solution. The reaction mixtures were incubated on ice for 2 h and then in Quenching Solution (1 M Tris∙HCl, pH 7.5) to a final concentration of 20–50 mM. The samples were then incubated for 15 min to quench non-reacted crosslinker and byproducts. SPDP was added to the protein samples to achieve a 4-fold molar excess of SPDP and was incubated for 60 min at room temperature. Excess of non-reacted crosslinker and reaction byproducts were removed by extensive dialysis.
The isolated membranes were then solubilized in HMS buffer containing 1% of DDM. Immunoprecipitation was performed with an antibody against PetB as described in . Afterwards, base-cleavage of BSOCOES was performed by increasing the pH of the solution containing the BSOCOES conjugate to 11.6 with NaOH, which was incubated for 2 h at 37 °C, but cross-links created using SPDP reagents can be cleaved with 25 mM DTT at pH 4.5 Obtained samples were dialyzed into HMS buffer for subsequent analysis. The proteins were then identified with MS.
Preparation of stroma fractions without the cpSRP54 protein
Protein A Sepharose CL-4B was prepared according to the standard protocol of GE Healthcare. An antibody against cpSRP54 (5 μg mL−1) was incubated 30 min at room temperature with 2 ml of isolated stroma fraction. Subsequently, 20 μL of protein A-Sepharose CL-4B resin in 20 mM sodium phosphate buffer, pH 7.0 was added and the incubation was continued with very mild rotation at 4 °C for an additional 90 min. The resin was collected by centrifugation at 500×g, 5 min, 4 °C and the supernatant fraction containing stromal proteins without cpSRP54 was collected and immediately used in a PetD insertion experiment.
Mass spectroscopy analysis
For identification, the proteins were analyzed as described in [11, 28]. Tandem mass spectrometry experiments were carried out on a Q-Tof Premier mass spectrometer in conjunction with a nanoACQUITY UPLC system (Waters Corp) to obtain peptide information. Tandem mass spectrometry output lists of precursor and product ion information were used for NCBI database searching using the program Mascot Distiller (version 2.1, Matrix Science) .
In order to identify inserted proteins using mass spectroscopy following the insertion assays, the proteins were analyzed by SDS/PAGE and autoradiography. A gel band containing the radioactive polypeptide was excised. Moreover, at the same time, positive and negative control samples for mass spectroscopy analysis were prepared. As positive control, proteins from the molecular weight marker were used, while a piece of empty gel with no protein present was used as a negative control in order to determine any contamination that may have occurred during sample processing. Proteins were first extracted from polyacrylamide gels using electroelution (D-Tube, Novagene). Following electroelution, salts, SDS, and dye were removed by dialysis in D-Tube and the resulting proteins samples were then concentrated using an Amicon Ultra spin column.
All samples were analyzed by MALDI-TOF using the 4800 Plus MALDI-TOF/TOF™ Analyzer (Applied Biosystems) as well. Mass spectra for each gel slice were obtained in three replicates. Additionally, the proteins were digested by trypsin and analyzed by MS with PMF. The analysis procedure included washing, destaining, reduction and alkylation as described in .
Protein concentrations were determined with the BCA™ protein assay kit (Pierce). Following the normalization of protein concentrations, proteins were separated via Tricine/Tris SDS-PAGE [11, 38] and transferred to nitrocellulose membranes using a semidry blotting system .
For protein detection, the following antibodies were used: polyclonal antibodies against PetB (antibodies raised against the 10 C-terminal amino acids, IRKQGIFGPL, of pea PetB) , cpSecY (antibodies raised against the 21 C-terminal residues of CRAEIISQKYKNIELYDFDKY of pea cpSecY) , ALB3 (antibodies raised against the 50 amino acid, PLTKQQVESTLAMQNPQPKIKAIQERYAGNQERIQLETSRLYTQAGVNPL, of the stromal protein sequence located between the first and second transmembrane (TM) domains) . Antibodies were prepared in rabbits injected with the appropriate synthetic peptide (GenScript). The overexpressed and purified cpSRP54  and a 21-amino acid synthetic peptide (YPIFAQQGYENPREATGRIVCANC), corresponding to the highly conserved N-terminus of the mature pea PetA , were used to prepare chicken (IgY) anti-cpSRP54 and anti-PetA antibodies, respectively. Antiserum was purified using the Pierce™ Chicken IgY Purification Kit (Thermo Scientific) followed by affinity-purification method against the antigen (Thermo Scientific). IgY antibodies was labelled with horseradish peroxidase using EZ-Link™ Plus Activated Peroxidase Kit (Thermo Scientific).
For antibody preparation, the antigen affinity purification (GenScript) or AminoLink™ Immobilization Kit (Thermo Scientific) were used. Affinity purification permits isolation of antibodies against the immunizing antigen and thereby eliminates cross-reactivity (‘unintentional’ binding) that would be present in the unpurified serum. The results of cross-reactivity studies are shown in Additional file 2: Figure S2. Antibodies were evaluated based on yield after affinity purification and the analysis of the specific activity of the purified antibodies. The titers of antigen-specific antibodies were determined by ELISA in 96-well Immulon 2HB flat bottom microtiter plates using the immunogen (Thermo Scientific).
The PetD protein presence in membrane and in immunoprecipitated complexes was confirmed by autoradiography followed by MS analysis with PMF since the antibodies directed against the PetD protein are unavailable . Prior to autoradiography, gels were stained with Coomassie brilliant blue to confirm equal protein loading and then dried under vacuum. Importantly, we always used the same exposure time so that the image intensity was used as a consistent guide to the exposure required for subsequent autoradiography. Molecular masses were determined by comparison to molecular weight ladder (MW). For autoradiography, the MW bands were gently marked on the X-ray film (Kodak) with a pencil and the X-ray film was placed over the dry gel and exposed for 24 h. Quantitative densitometry of Western blot and autoradiography were performed using Image Lab software v. 4.1 (BioRad). The antibody bound proteins were detected by a chemiluminescence reaction using ECL (enhanced chemiluminescence, Amresco).
Bioinformatic analysis of PetD did not identify a membrane targeting signal sequence
Using TargetP tools, PetD was predicted to be a protein without a specific signal sequence or anchor sequence (Fig. 1d). Additionally, we used PredSL and PSORT software tools which combine multiple methods in order to predict a protein’s localization to the chloroplast and the thylakoids as well as to the mitochondrion or the secretory pathway . Unfortunately, neither of the predictive methods used could identify the presence of a signal sequence potentially responsible for PetD transfer and its insertion into the thylakoid membrane (Fig. 1d).
The N-terminal regions of membrane proteins often serve as a membrane anchor, whereas the majority of known membrane proteins are translocated into membranes by a cleavable or non-cleavable signal sequence. In order to detect a potential non-cleavable signal within the PetD primary structure, the Kyte–Doolittle analysis was performed with a sequence window size of 5 amino acids that is appropriate for detecting a potential hydrophobic signal at N-terminus. The resulting data were aligned relative to the TM2-L18-TM3 amino acid sequence of the light-harvesting chlorophyll a/b binding proteins subunit B (LHCB1) (Fig. 1a). During the post-translational cpSRP-dependent insertion of LHCP, LHCP affiliates with cpSRP54 and cpSRP43 to form a stromal ‘transit complex’. Formation of this complex is governing by a sequence of specific recognition and interaction events. The interaction between LHCP and cpSRP43 involves a conserved 18 amino acid span, termed L18, that is located between two TMH, (TM2 and TM3) of LHCP. Hence, the L18 provides an internal targeting signal for a highly specific interaction between LHCPs and cpSRP43. After transit complex assembly, a third protein, cpFtsY, is assumed to target the transit complex to the thylakoid membrane .
The results of the Kyte–Doolittle analysis indicate that PetD does not contain a non-cleavable signal sequence. However, as shown in Fig. 1b, the average hydropathy plots of the compared PetD and LHCB1 sequences show similar profiles within the first 40 amino acids, but at a different hydrophobicity level. A consensus amino acid sequence, PLEIL (ankyrin repeats motive), is present in both proteins. Figure 1c shows the topography of the PLEIL sequence, marked in blue in the PetD structure . However, the PetD sequence is missing DPLG motif that is responsible for cpSRP43 binding by LHCB1 . Moreover, the average hydropathicity (GRAVY) values of these PLEIL sequences were similar (0.817 and 0.989 respectively).
For the most of the outer envelope proteins, their targeting plastid compartments (inner envelope membrane, stroma, and thylakoid) depend on the presence of a cleavable transit sequence in the precursor N-terminal region. Furthermore, the reports of Miras, et al.  suggest that neither the N-terminal nor the C-terminal sequences are essential for chloroplastic membrane localization of the ceQORH (Chloroplast Envelope Quinone Oxidoreductase Homologue) that has non-canonical sequence for chloroplast membrane integration. Therefore, we compared the PetD sequence with the non-canonical non-cleavable transit peptides of ceQORH. These analyses did not identify any sequence similarities between PetD and ceQORH within the range of 75 N-terminal amino acids. However, the Kyte–Doolittle hydropathy profiles of the first 75 amino acid of PetD and ceQORH protein (Additional file 3: Figure S4A) show some similarities between first 35 amino acid region of the PetD amino acid sequence is shifted by 8 amino acids toward the C-terminal end (Additional file 3: Figure S4B).
PetD is not inserted directly into the membrane in an unassisted posttranslational manner
To verify that the PetD protein integration into the pea thylakoid membrane can occur by the spontaneous pathway, the protein was expressed in a cell-free system as described in  in the presence of proteinase K-treated thylakoid membranes, but in the absence of the stromal fraction. During this translation protocol, 30% of the synthesized PetD protein was associated with the thylakoid membrane fraction (Fig. 2a). The molecular weight of inserted and integrated PetD protein was determined by mass spectrometry (Additional file 4: Figure S5A). MS analysis confirmed that the PetD construct is correctly expressed in the in vitro translation system. The molecular weight observed for PetD (17,487 Da) in MS analysis agrees well with the theoretical molecular weight of the monomeric species (17,476 Da).
The PetD protein integration with thylakoid membrane was further tested with use of strong chaotropic salts like NaBr and NaSCN that remove proteins peripherally bound to the membrane without membrane disassembly [32, 44, 45]. As shown in Fig. 2b, the membrane associated PetD was almost completely removed. Moreover, in order to facilitate spontaneous membrane integration, the synthesized PetD protein was solubilised in the presence of 0.2% DDM, and then mixed with purified thylakoid membrane. This approach, however, did not increase the resistance of the membrane-associated PetD to chaotropic removal (Fig. 2c). As a control for spontaneous protein insertion, we tested whether PsbW of the photosystem II or PetB can be spontaneously inserted into the thylakoid membrane [11, 46]. Both control experiments were performed under the same conditions as those for the petD protein. These experiments confirmed the previous observation that PsbW is indeed inserted into the thylakoid membranes by the spontaneous pathway. Furthermore, we also confirmed that PetB cannot be spontaneously inserted into thylakoid membranes (Additional file 5: Figure S6). All above results indicate that the native PetD cannot incorporate into thylakoid membranes spontaneously.
Post-translation import of PetD into the thylakoid membrane is SRP-dependent
The previous comparative analysis of ribosomal fractions bound to thylakoid membranes and from stroma indicate that PetD is translated on free ribosomes exclusively , suggesting a post-translational mechanism for protein membrane integration [12, 22]. Therefore, since the results of the thylakoid import assays excluded the spontaneous pathway for PetD membrane integration, we next verified the proposed post-translational translocation of PetD using a cell free in vitro system.
PetD expression was performed in a cell-free system by using the transcription–translation procedure where transcription and translation reaction were separated in space and time (see Materials and Methods, section: The cell free transcription-translation insertion assay). Moreover, during the post-translational insertion experiments, translations were performed in the absence of thylakoid membranes. As shown in Fig. 3a, without the stroma fraction the translation product (PetD) did not integrate into the membranes and was detected in the supernatant only. However, when the stroma fraction was present together with the membranes, the PetD was localized in the membrane fraction (Fig. 3b). Importantly, both inserted PetD and PetA (control) are resistant against all extraction procedures (Fig. 3c and f) . Moreover, this result clearly shows that proper PetD membrane incorporation occurs via the post-translational mechanism and requires the stroma factors.
Hence using specific antibodies, we examined the mechanisms responsible for the membrane integration of PetD. Pretreatment of the thylakoids membrane with Ab against ALB3 prevented insertion of PetD into the membranes (Fig. 3d), suggesting that ALB3 is required for this process. However, although a significant level of PetD insertion was achieved in the presence of cpSecY Ab (Fig. 3e), it was still lower than in the control (Fig. 3e). Hence, the Ab against cpSecY only partially prevents cpSecA-dependent translocation of PetD into the membrane by the Sec pathway since this antibody blocks cpSecA binding to cpSecY without functional inhibition of cpSecY [39, 47, 48].
The current model of cytochrome b 6 f complex assembly assumes the partial transcriptional activation of psbBNH-petBD operon within the PetB and PetD genes . Following transcription, the PetB and PetD mRNA are translated into the polypeptides. As a result of this process, both proteins are incorporated into the membrane and form the polytopic monomeric core of the cytochrome b 6 f complex. Hence, in order to validate the proposed mechanism for formation of PetD-PetB core complex, both genes were transcribed/translated in the cell-free system in the presence of the stroma and thylakoid membranes fractions (see PetD and PetB co-expression in cell-free assay in Materials and Methods section). After 1 h of translation, a membrane permeable and cleavable crosslinker (BSOCOES) were added. As shown in Fig. 4, the cotranslation of PetB and PetD resulted in detection of the PetD translation product with higher molecular weight than compared to PetD translated alone (Fig. 4a). Furthermore, the immunoprecipitation with an Ab against PetB of cross-linked isolated membrane complexes, followed by MALDI-TOF mass analysis confirmed that the radiolabeled complex contains a PetD-PetB complex (Fig. 4b and Additional file 4: Figure S5B).
As shown in Fig. 5, when the posttranslational insertion of PetD was performed in the presence of thylakoid membrane and stroma fractions devoid of the cpSRP54 protein, the PetD protein did not incorporate into membranes. Although a very weak signal is observed (Fig. 5a), it probably results from the incomplete removal of the cpSRP54 from stroma (as shown in Fig. 5b). Furthermore, two control experiments were set up to ensure the integrity of the method. In the first experiment, shown in Fig. 5c, we tested the co-translational insertion of PetB in the presence or absence of the cpSRP54 protein. As expected, we did not observe PetB insertion in the absence of the cpSRP54 protein. The goal of the second experiment (Fig. 5d) was to determine the impact of using a different concentration of antibody against cpSrp54 during PetD posttranslational insertion. We found that with increasing concentrations of antibody, there was corresponding decrease in the level of PetD embedded in the thylakoid membrane.
Furthermore, to identify the chloroplast proteins that governed PetD import, a chemical cross-linking analysis followed by immunoprecipitation using antibodies directed against ALB3 or cpSEC54 was performed followed by MS detection. The results of immunoprecipitation analysis combined with MS-PMF and Mascot Distiller analysis are shown in Table 1. The results show the association of cpSRP, cpFtsY and ALB3 proteins with PetD during its insertion into the membrane. Furthermore, the analysis resulted in the identification of several hundred of peptides that could not be assigned to specific proteins or to proteins with low score (Additional file 6: Table S1).
These MS results were further confirmed by Western blot analyses of the cross-linked complexes followed by immunochemistry. As presented in Fig. 6a, the Ab against cpSRP54 detected large amounts of cpSRP54 in the isolated membrane fractions. A smaller, but significant pool of cpSRP54 was detected in the immobilized ALB3 antibody unbound fraction as well. Importantly, the immunopurification of the cross-linked PetD complexes with antiserum directed against the cpSRP54 (Fig. 6b) allowed for the detection of ALB in the purified PetD complexes.
The proteins we identified by MS were previously recognized as crucial for SRP-mediated post-translational transport of LHC proteins into the thylakoid membrane . Hence, our results of MS (Table 1) and autoradiograph analyses (Fig. 3) are strong indicators that PetD import into the membrane occurs through the post-translational SRP pathway.
MALDI-TOF mass analysis (Fig. 7) of intact proteins crosslinked by SPDP, immunoprecipitated and then cleaved with 25 mM DTT from the petD complexes shows two of CBB proteins (CCB1 and CCB3) that play important roles in guidance of apocytochromes and in haem groups for their covalent linkage by the cytochrome-c-hem lyase . Importantly this analysis confirmed that after insertion PetD and PetB form a complex at the membrane with predicted size of 38.67 kDa (Fig. 4a, Table 2 and Additional file 4: Figure S5B). This result was confirmed by MS analysis of a tryptic digested PetD complex (Table 2).
In chloroplasts, there is significant intraorganellar sorting of proteins, and because of the endosymbiotic origin of chloroplasts, the process of translocating a protein from the stroma of the chloroplast to the thylakoid lumen and membrane is topologically equivalent to prokaryotic protein secretion [50, 51]. The related signals are analogous to the SP for protein secretion. The signals are organized in a bipartite presequence with the cTP at the N-terminus, followed by the SP-like signal, which is resolved only after the processing of the cTP fragment. Furthermore, the luminal transfer peptide (lTP) that is present in proteins translocated to the thylakoid lumen, is very similar to the SP signal .
Over the past decade, several computational methods have been developed to predict subcellular localization of plant proteins . Gómez, et al.  have shown that the SignalP software, using Gram-negative bacteria settings (TargetP contains SignalP prediction algorithm), correctly predicts the processing site for proteins that are imported into the thylakoid membrane via the “spontaneous” pathway. This observation suggests that the mechanism of spontaneous import of thylakoid protein into membrane may be related to the mechanism of substrate secretion in Gram-negative bacteria. However, our analysis shows that TargetP and PredSL suggested “other” locations for the PetD protein. Only the PSORT software identified the chloroplast thylakoid membrane as a possible targeting location for PetD, but the score of this analysis was low. Hence, it can be concluded that none of the used programs could convincingly predict the transit peptide processing site of PetD.
Because PetD lacks a canonical signal sequence for protein translocation and membrane integration, we examined PetD primary structure for other non-canonical motifs that may govern its membrane import. The PetD sequence was compared with non-canonical non-cleavable transit peptides of ceQORH. These analyses did not identify sequence similarities between PetD and ceQORH. However, when we compared both these sequences, we observed some similarity with the ceQORH sequence in their hydrophobic and hydrophilic properties. Concluding from this analysis about signal or anchor sequences, however, would be speculative at best. Therefore, further studies will be required to understand the role of the petD N-terminal sequence during the membrane integration process. Furthermore, Miras, et al.  suggested that a domain of the ceQORH bacterial ancestor may have evolved so as to exclude the general requirement for an N-terminal plastid transit sequence.
The PetD primary structure is related to amino acid sequence TM2-L18-TM3 of LHCB1 that is responsible for cpSRP43 binding (Fig. 1a). The LHCB1-cpSRP43 interaction is predominantly hydrophobic and a DPLG motif between the TM2 and TM3 is known to be required for the binding with cpSRP43 . Although, the hydropathy profiles of amino acids between the second and the third helix of PetD and LHCB1 are very similar, PetD lacks the DPLG consensus sequence. The lack of a DPLG motif in the PetD sequence suggests that this protein does not directly bind cpSRP43. However, the PLEIL motif may mediate protein-protein interactions as well and therefore limit the need for this other motif .
Because the bioinformatic analyses did not identify the PetD sequence responsible for directing this protein to the appropriate insertion pathway, we first determined if PetD can be spontaneously integrated into the thylakoid membrane. We performed a comparative analysis of PetD insertion into purified proteinase K-treated thylakoid membranes. Our criteria for determining the correct mechanism of insertion were that the protein should be integrated within the membrane and therefore resistant to chaotropic extraction. As a control, we tested if PsbW that is integrated into the thylakoid membrane by a spontaneous mechanism and PetB protein that is integrated into the thylakoid membrane in a co-translational manner are resistant to chaotropic extractions. Our results indicated that chaotropic agents easily extracted PetD from the thylakoid membrane, whereas both control proteins were resistant to chaotropic extraction. Since the native PetD protein is resistant to chaotropic agent’s extraction , our results exclude spontaneous insertion of PetD into the thylakoid membrane.
PetD is one of the major chloroplast-encoded subunits of cytochrome b 6 f and has been proposed by previous studies to be post-translationally integrated with thylakoid membrane [12, 22]. However, this hypothesis was never verified. Therefore, the aim of this study was to identify the mechanisms responsible for PetD thylakoid membrane integration using a cell free transcription/translation system in different experimental conditions (in the presence of thylakoid membranes or thylakoid membranes and stroma proteins fraction or without thylakoid membranes) .
The analysis of the ribosomal fraction binding with thylakoid membranes and from stroma indicated that PetD is translated from the dicistronic PetB–PetD transcript on free ribosomes exclusively . Also, we confirmed that PetD was not found in the membrane bound ribosomes and was predominantly synthesized by free ribosomes (Additional file 7: Figure S7). Hence, we assume the possibility of a strictly post-translational integration of PetD into the thylakoid membrane by the GTP-dependent SRP pathway. Inspection of our data demonstrate unequivocally that the post-translationally inserted PetD is resistant to chaotropic extraction like a native PetA and suggests that PetD is integrated and anchored in the membrane by strong electrostatic and hydrophobic forces.
In order to verify this hypothesis, we followed the PetD thylakoid membrane integration in vitro. Furthermore, to identify chloroplast proteins that could govern PetD import, a chemical cross-linking protocol followed by immunoprecipitation approach combined with mass spectrometry detection was applied. Using this approach, we show that two chloroplast GTPases, cpSRP54 and cpFtsY and the chloroplast translocase ALB3, are involved in the PetD membrane integration process. cpFtsY is a GTPase receptor that is partitioned between the target membrane and the soluble phase and its function in thylakoid biogenesis is to target integral membrane proteins to thylakoids. Moreover, cpFtsY plays a critical role in the intramolecular communication that regulates cpSRP receptor functions at the membrane . During the posttranslational targeting the preserves hydrophobic substrates in an integration competent form. At the membrane, the substrate is identified by the translocase and consequently discharged from the cpSRP54 for integration . Importantly, during the import assay, only the Ab against ALB3  completely prevented insertion of PetD into the thylakoid membrane. Hence, blocking ALB3 association with cpSRP and cpFtsY and inhibiting PetD protein integration suggest that formation of a protein complex containing cpSRP, its receptor, cpFtsY, and ALB3 translocase is required for proper PetD integration . However, use of antibodies against cpSecY only partially limited PetD membrane import [9, 39]. Although, these results suggest that cpSecY is involved in membrane integration of PetD, the limited inhibition of PetD integration in this case may be due to other factors not related to protein transport and incorporation of proteins into the thylakoid membrane. Moreover, immunoprecipitation of cross-linked proteins with an Ab against ALB3 or cpSEC54 combined with MS and PMF analysis performed in the presence of the stroma fraction detected cpSRP43 to be only “associated” with cpSRP54-PetD complexes. However, we do not observe this protein in the isolated membrane bound cpSRP54-PetD complexes.
The SRP complexes in the chloroplast stroma, heterodimers consisting of cpSRP43 and cpSRP54, are observed in plants only [16, 18] and responsible for LHCPs import into the thylakoid membrane . Dünschede, et al.  suggested that the coevolution of LHCPs and cpSRP43 developed autonomously of the complex arrangement with cpSRP54, and that the interaction between cpSRP54 and cpSRP43 developed more recently during the progression from chlorophytes to land plants. Importantly, stroma also contains a free cpSRP54 fraction that is not bound with cpSRP43, and that can bind to the 70S ribosome and participate in cotranslational integration of membrane proteins . Hence, our data suggest that PetD membrane integration is mediated mainly by cpSRP54.
Based on the results of the analysis by mass spectrometry, we cannot exclude that cpSRP43 is involved in this process before the cpSRP54-PetD complex formation. However, since PetD lacks the DPLG consensus, the cpSRP43 presence in the stroma fraction may be an experimental artifact as well. Although, Dünschede, et al.  demonstrated that the cpSRP system in Chlamydomonas reinhardtii differs from that of land plants in that cpSRP43 is complexed to cpSRP54, Tzvetkova-Chevolleau, et al.  showed that cpSRP43’s role in targeting LHCPs to the thylakoid membranes is independent of cpSRP54/cpFtsY. Hence, the possible involvement of cpSECY insertase in the PetD membrane import process will also requires further study.
Interestingly, we detected two cofactor assemblies on complex C protein subunits, CCB1 and CBB3 which are involved in PetB biogenesis, in double expression assays of isolated crosslinked PetD-PetB complexes that were analyzed by MS  (Additional file 8: Table S2). Additional file 9: Figures S8 and S9 show the sequence alignment of CCB1 and CCB3. After its insertion into the thylakoid membrane, apocytochrome b 6 binds its two non-covalent b-type haems . The integration of both haems to apocytochrome b 6 occurs spontaneously and does not require adjuvant proteins involvement [62, 63]. However, the consequent binding of haem c i via an untypical single thioether bond engages an enzymatic CCB system .
Herein, we propose the cpSRP54-cpFtsY-ALB3-based transport mechanism (Fig. 8) that mediates the import of PetD protein into the thylakoid membranes. Furthermore, we show that the PetD integration into thylakoid membrane is post-translational and an SRP-dependent process that relies on cpSRP54-cpFtsY-ALB3-PetD complex formation. This machinery may require an atypical signal sequence, that we cannot identify using the current algorithms. Furthermore, we cannot exclude the possibility that cpSRP43 proteins do not “participate” in this process.
Na-benzoyl-arginine ethyl ester
bovine serum albumin
N-hydroxysuccinade ester bis[2-(succinimidyloxycarbonyloxy) ethyl] sulfone
chloroplast transit peptides
subtype light-harvesting chlorophyll a/b binding proteins
light-harvesting chlorophyll a/b-binding proteins
lumenal transfer peptide
Q-TOF mass spectroscopy
mitochondrial targeting peptide
cytochrome b 6
peptide mass fingerprints
subunit W of PS II
ribosome nascent chain complexes
sodium dodecyl sulphate
sodium dodecyl sulphate polyacrylamide gel electrophoresis
signal recognition particle
Cramer WA, Soriano GM, Ponomarev M, Huang D, Zhang H, Martinez SE, Smith JL. Some new structural aspects and old controversies concerning the cytochrome b6f complex of oxygenic photosynthesis. Annu Rev Plant Physiol Plant Mol Biol. 1996;47:477–508.
Hope AB. The chloroplast cytochrome bf complex: a critical focus on function. Biochim Biophys Acta. 1993;1143(1):1–22.
Li L-B, Zou Y-P, Yu L, C-A Y. The catalytic role of subunit IV of the cytochrome b6-f complex from spinach chloroplast. Biochim Biophys Acta. 1991;1057(2):215–22.
Santillán Torres JL, Atteia A, Claros MG, González-Halphen D. Cytochrome f and subunit IV, two essential components of the photosynthetic bf complex typically encoded in the chloroplast genome, are nucleus-encoded in Euglena Gracilis. Biochim Biophys Acta. 2003;1604(3):180–9.
Chi W, Ma J, Zhang L. Regulatory factors for the assembly of thylakoid membrane protein complexes. Philos Trans R Soc Lond Ser B Biol Sci. 2012;367(1608):3420–9.
Kuras R, de Vitry C, Choquet Y, Girard-Bascou J, Culler D, Büschlen S, Merchant S, Wollman F-A. Molecular genetic identification of a pathway for Heme binding to cytochrome b6. J Biol Chem. 1997;272(51):32427–35.
Aldridge C, Spence E, Kirkilionis MA, Frigerio L, Robinson C. Tat-dependent targeting of Rieske iron-sulphur proteins to both the plasma and thylakoid membranes in the cyanobacterium Synechocystis PCC6803. Mol Microbiol. 2008;70(1):140–50.
Aldridge C, Cain P, Robinson C. Protein transport in organelles: protein transport into and across the thylakoid membrane. FEBS J. 2009;276(5):1177–86.
Schuenemann D. Mechanism of protein import into thylakoids of chloroplast. Biol Chem. 2007;388:907–15.
Komar AA. A pause for thought along the co-translational folding pathway. Trends Biochem Sci. 2009;34(1):16–24.
Kroliczewski J, Piskozub M, Bartoszewski R, Kroliczewska B. ALB3 Insertase mediates cytochrome b6 co-translational import into the thylakoid membrane. Sci Rep. 2016;6:34557.
Zoschke R, Barkan A. Genome-wide analysis of thylakoid-bound ribosomes in maize reveals principles of cotranslational targeting to the thylakoid membrane. Proc Natl Acad Sci U S A. 2015;112(13):E1678–87.
Chatzi KE, Sardis MF, Economou A, Karamanou S. SecA-mediated targeting and translocation of secretory proteins. Biochim Biophys Acta. 2014;1843(8):1466–74.
Mitra K, Frank J, Driessen A. Co- and post-translational translocation through the protein-conducting channel: analogous mechanisms at work? Nat Struct Mol Biol. 2006;13(11):957–64.
DeLille J, Peterson EC, Johnson T, Moore M, Kight A, Henry R. A novel precursor recognition element facilitates posttranslational binding to the signal recognition particle in chloroplasts. Proc Natl Acad Sci U S A. 2000;97(4):1926–31.
Schuenemann D, Gupta S, PerselloCartieaux F, Klimyuk VI, Jones JDG, Nussaume L, Hoffman NE. A novel signal recognition particle targets light-harvesting proteins to the thylakoid membranes. Proc Natl Acad Sci U S A. 1998;95(17):10312–6.
Franklin AE, Hoffman NE. Characterization of a chloroplast homologue of the 54-kDa subunit of the signal recognition particle. J Biol Chem. 1993;268(29):22175–80.
Kathir KM, Rajalingam D, Sivaraja V, Kight A, Goforth RL, Yu C, Henry R, Kumar TK. Assembly of chloroplast signal recognition particle involves structural rearrangement in cpSRP43. J Mol Biol. 2008;381(1):49–60.
Stengel KF, Holdermann I, Wild K, Sinning I. The structure of the chloroplast signal recognition particle (SRP) receptor reveals mechanistic details of SRP GTPase activation and a conserved membrane targeting site. FEBS Lett. 2007;581(29):5671–6.
Stroebel D, Choquet Y, Popot JL, Picot D. An atypical haem in the cytochrome b(6)f complex. Nature. 2003;426(6965):413–8.
Zak E, Sokolenko A, Unterholzner G, Altschmied L, Herrmann RG. On the mode of integration of plastid-encoded components of the cytochrome bf complex into thylakoid membranes. Planta. 1997;201(3):334–41.
Friemann A, Hachtel W. Chloroplast messenger RNAs of free and thylakoid-bound polysomes from Vicia Faba L. Planta. 1988;175(1):50–9.
Emanuelsson O, Nielsen H, Heijne GV. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. Protein Sci. 1999;8(5):978–84.
Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982;157(1):105–32.
Katoh K, Standley DM. MAFFT Multiple Sequence Alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30(4):772–80.
Petsalaki EI, Bagos PG, Litou ZI, Hamodrakas SJ. PredSL: a tool for the N-terminal sequence-based prediction of protein subcellular localization. Genomics, Proteomics, Bioinformatics. 2006;4(1):48–55.
Nakai K, Kanehisa M. A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics. 1992;14(4):897–911.
Piskozub M, Kroliczewska B, Kroliczewski J. Ribosome nascent chain complexes of the chloroplast-encoded cytochrome b6 thylakoid membrane protein interact with cpSRP54 but not with cpSecY. J Bioenerg Biomembr. 2015;47(3):265–78.
Cline K, Henry R, Li CJ, Yuan JG. Multiple pathways for protein transport into or across the thylakoid membrane. EMBO J. 1993;12(11):4105–14.
Arnon D. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiol. 1949;24(1):1–15.
Margulies MM, Tiffany HL, Hattori T, Photosystem I. Reaction center polypeptides of spinach are synthesized on thylakoid-bound ribosomes. Arch Biochem Biophys. 1987;254(2):454–61.
Houben E, de Gier JW, van Wijk KJ. Insertion of leader peptidase into the thylakoid membrane during synthesis in a chloroplast translation system. Plant Cell. 1999;11(8):1553–64.
Karnauchov I, Herrmann RG, Klosgen RB. Transmembrane topology of the Rieske Fe/S protein of the cytochrome b6/f complex from spinach chloroplasts. FEBS Lett. 1997;408(2):206–10.
Michl D, Robinson C, Shackleton JB, Herrmann RG, Klosgen RB. Targeting of proteins to the thylakoids by bipartite presequences: CFoII is imported by a novel, third pathway. EMBO J. 1994;13(6):1310–7.
Bonifacino JS, Dell'Angelica EC, Springer TA. Immunoprecipitation. Curr Protoc Protein Sci. 2001;
Perkins DN, Pappin DJ, Creasy DM, Cottrell JS. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999;20(18):3551–67.
Webster J, Oxley D. Protein identification by MALDI-TOF mass spectrometry. In: Zanders ED, Totowa NJ, editors. Chemical genomics and proteomics: reviews and protocols: Humana Press; 2012. p. 227–40.
Schagger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987;166(2):368–79.
Mori H, Summer EJ, Ma X, Cline K. Component specificity for the thylakoidal sec and Delta pH-dependent protein transport pathways. J Cell Biol. 1999;146(1):45–56.
Moore M, Harrison MS, Peterson EC, Henry R. Chloroplast Oxa1p homolog Albino3 is required for post-translational integration of the light harvesting chlorophyll-binding protein into thylakoid membranes. J Biol Chem. 2000;275(3):1529–32.
Smith MD, Licatalosi DD, Thompson JE. Co-Association of Cytochrome f catabolites and plastid-lipid-associated protein with chloroplast lipid particles. Plant Physiol. 2000;124(1):211–22.
CJ T, Peterson EC, Henry R, Hoffman NE. The L18 domain of light-harvesting chlorophyll proteins binds to chloroplast signal recognition particle 43. J Biol Chem. 2000;275(18):13187–90.
Miras S, Salvi D, Ferro M, Grunwald D, Garin J, Joyard J, Rolland N. Non-canonical transit peptide for import into the chloroplast. J Biol Chem. 2002;277(49):47770–8.
Breyton C, de Vitry C, Popot JL. Membrane association of cytochrome b6f subunits. The Rieske iron-sulfur protein from Chlamydomonas Reinhardtii is an extrinsic protein. J Biol Chem. 1994;269(10):7597–602.
Ohlendieck K: Extraction of membrane proteins. In: Protein Purification Protocols. Edited by Cutler P, 2 edn. New York: Humana Press, Totowa; 2003: 283–290.
Woolhead CA, Mant A, Kim SJ, Robinson C, Rodger A. Conformation of a purified "spontaneously" inserting thylakoid membrane protein precursor in aqueous solvent and detergent micelles. J Biol Chem. 2001;276(18):14607–13.
Jiang F, Yi L, Moore M, Chen M, Rohl T, Van Wijk KJ, De Gier JW, Henry R, Dalbey RE. Chloroplast YidC homolog Albino3 can functionally complement the bacterial YidC depletion strain and promote membrane insertion of both bacterial and chloroplast thylakoid proteins. J Biol Chem. 2002;277(22):19281–8.
Schuenemann D, Amin P, Hartmann E, Hoffman NE. Chloroplast SecY is complexed to SecE and involved in the translocation of the 33-kDa but not the 23-kDa subunit of the oxygen-evolving complex. J Biol Chem. 1999;274(17):12177–82.
Saif Hasan S, Yamashita E, Cramer WA. Transmembrane signaling and assembly of the cytochrome b6f-lipidic charge transfer complex. Biochim Biophys Acta. 2013;1827(11–12):1295–308.
Emanuelsson O, Brunak S, von Heijne G, Nielsen H. Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protocols. 2007;2(4):953–71.
McFadden GI, van Dooren GG. Evolution: red algal genome affirms a common origin of all plastids. Curr Biol. 2004;14(13):R514–6.
Imai K, Nakai K. Prediction of subcellular locations of proteins: where to proceed? Proteomics. 2010;10(22):3970–83.
Gómez SM, Bil’ KY, Aguilera R, Nishio JN, Faull KF, Whitelegge JP. Transit peptide cleavage sites of integral thylakoid membrane proteins. Mol Cell Proteomics. 2003;2(10):1068–85.
Xu C, Jin J, Bian C, Lam R, Tian R, Weist R, You L, Nie J, Bochkarev A, Tempel W, et al. Sequence-specific recognition of a PxLPxI/L motif by an ankyrin repeat tumbler lock. Sci Signal. 2012;5(226):ra39.
Marty NJ, Rajalingam D, Kight AD, Lewis NE, Fologea D, Kumar TK, Henry RL, Goforth RL. The membrane-binding motif of the chloroplast signal recognition particle receptor (cpFtsY) regulates GTPase activity. J Biol Chem. 2009;284(22):14891–903.
Eichacker LA, Henry R. Function of a chloroplast SRP in thylakoid protein export. Biochim Biophys Acta. 2001;1541(1):120–34.
Moore M, Goforth RL, Mori H, Henry R. Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids: substrate not required. J Cell Biol. 2003;162(7):1245–54.
Richter CV, Bals T, Schunemann D. Component interactions, regulation and mechanisms of chloroplast signal recognition particle-dependent protein transport. Eur J Cell Biol. 2010;89(12):965–73.
Dünschede B, Träger C, Schröder CV, Ziehe D, Walter B, Funke S, Hofmann E, Schünemann D. Chloroplast SRP54 Was Recruited for Posttranslational Protein Transport via Complex Formation with Chloroplast SRP43 during Land Plant Evolution. J Biol Chem. 2015;290(21):13104–14.
Tzvetkova-Chevolleau T, Hutin C, Noël LD, Goforth R, Carde J-P, Caffarri S, Sinning I, Groves M, Teulon J-M, Hoffman NE, et al. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell. 2007;19(5):1635–48.
Saint-Marcoux D, Wollman F-A, de Vitry C. Biogenesis of cytochrome b6 in photosynthetic membranes. J Cell Biol. 2009;185(7):1195–207.
Dreher C, Prodohl A, Hielscher R, Hellwig P, Schneider D. Multiple step assembly of the transmembrane cytochrome b6. J Mol Biol. 2008;382(4):1057–65.
Kroliczewski J, Szczepaniak A. In Vitro reconstitution of the spinach chloroplast cytochrome b6 protein from a fusion protein expressed in Escherichia Coli. Biochim Biophys Acta. 2002;1598(1–2):177–84.
Humann J, Jung S, Zheng P, Cheng C-H, Lee T, Frank M, McGaughey D, Scott K, Yu J, Ficklin S et al: Cool Season Food Legume Genome Database: An up-to-date resource enabling genetics, genomics and breeding research in pea, lentil, faba bean and chickpea. In: Plant & Animal Genome Conference XXIV: 2016; San Diego, CA, USA.; 2016.
We would like to thank Prof. James Collawn for critical reading of the manuscript, Prof Matthias Rögner for help during plasmid pET25b-SUIV construction, and Małgorzata Piskozub for her help in antibodies preparation. We would also like to acknowledge Prof Andrzej Szczepaniak for some kind help and laboratory support in early stages of this work.
This work was partially supported by grants NN301464434 from the Ministry of Science and Higher Education to J.K. The funding body did not play a role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript, but just provide the financial support. The cost of publication fee was supported by Wroclaw Centre of Biotechnology Programme, The Leading National Research Centre (KNOW) for years 2014–2018 and had no influence over the experimental design, data analysis or interpretation, or writing the manuscript.
Availability of data and materials
The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
The plant materials were purchased from Przedsiębiorstwo Nasiennictwa Ogrodniczego i Szkółkarstwa Ożarów Mazowiecki (PNOS, Poland) and commercially destined for sowing. Therefore, the use of collection of plant materials did not require permission.
Consent for publication
The authors declare no competing financial interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
The pT7CFE1-CHis which is optimized to use with the 1-Step Human In Vitro Protein Expression System. (PDF 165 kb)
Western blot testing for cross-reaction of the anti-PetB, anti-cpSrp54 or ALB3 antibodies with proteins extracted from chloroplast. (PDF 195 kb)
The Kyte–Doolittle hydropathy profile of the first 75 amino acids of pea PetD and ceQORH (Chloroplast Envelope Quinone Oxido-Reductase Homologue). (PDF 146 kb)
MALDI-TOF mass spectra. (PDF 112 kb)
Analyses of thylakoid membrane fractions and stroma after insertion of PetB or PsbW protein by spontaneous pathway. (PDF 121 kb)
Analysis of proteins co-immunoprecipitated with PetD after in vitro translation followed by posttranslational insertion into thylakoid membrane. (PDF 119 kb)
Autoradiograph of isolated free and membrane bound ribosomes isolated during cell-free expression of PetD. (PDF 235 kb)
Analysis of proteins co-immunoprecipitated with PetD-cytochrome b 6 complexes after in vitro translation followed by insertion into thylakoid membrane. (PDF 140 kb)
Sequence alignment of CCB1 and CCB3 proteins. (PDF 122 kb)
Sequence alignment of PetD protein. (PDF 351 kb)
About this article
Cite this article
Króliczewski, J., Bartoszewski, R. & Króliczewska, B. Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane. BMC Plant Biol 17, 213 (2017). https://0-doi-org.brum.beds.ac.uk/10.1186/s12870-017-1176-2
- Cytochrome b 6 f complex
- Thylakoid protein import