Fig. 4

Analysis of PetD and cytochrome b ₆ co-insertion into the thylakoid membrane. a Autoradiograph of thylakoid membrane fractions are shown after insertion of radiolabeled PetD and cytochrome b ₆ co-expressed in cell-free assay. b Analysis of isolated high molecular weight PetD crosslinking complex is shown after immunoprecipitation with antibody against cytochrome b ₆ and cleavage of crosslinker (BSOCOES). The interacting proteins were identified using MS with Mascot Distiller analysis. These proteins were confirmed as PetD and PetB. Moreover, the analysis resulted in the identification of a number of peptides that were impossible to assign to a specific protein, as well as proteins with very low total score [36]. The molecular weight of the autoradiography band was determined by MALDI-TOF. For size detection, the molecular weight ladder bands were marked on the X-ray films with a pencil