Fig. 5
From: Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane
PetD insertion into the thylakoid membrane after removal of the cpSRP54 protein. Analysis of PetD insertion in the presence of thylakoid membrane and stroma fraction after the cpSRP54 protein (denoted with “-”) was removed by immunoprecipitation. a Autoradiograph of thylakoid membrane fractions after radiolabeled PetD was inserted in the presence of thylakoid membrane and stroma fraction (M) with or without the cpSRP54 protein; TMi -total translation mixture. The MW of radiolabeled product was determined by MS. b Western blot analysis of the stroma fraction is shown with or without the cpSRP54 protein. MW- molecular weight marker; A- stroma fraction used in experiment and stroma fractions with or without the cpSRP54 protein (control). c Autoradiograph of thylakoid membrane fractions are shown after insertion of radiolabeled PetB according to the method described in [11]. PetB insertion in the presence of thylakoid membrane and stroma fraction with (control); without the cpSRP54 protein and after cpSRP54 recovery to stroma fraction; total translation mixture (TMi) was used as a control; d Autoradiograph of thylakoid membrane fractions are shown after insertion of radiolabeled PetD in the presence of thylakoid membrane and stroma fractions. Prior to the experiment, the stroma protein fraction was incubated with different concentrations of antibody against cpSRP54 for 4 h at room temperature with gentle mixing. The total translation mixture (TMi) without antibody was used as a control