Fig. 6
From: Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane
Immunoblotting analysis of the cross-linked PetD complexes after affinity purification. a The fractions were immunoprecipitated with antiserum directed against the ALB3 and then blotted for cpSRP54. the total translation mixture (TMi) was used as a control; b The fractions were immunoprecipitated with antiserum directed against the cpSRP54 and then blotted for ALB3. Total translation mixture (TMi) was used as a control. Both experiments were twice repeated. An aliquot of 15 μg of total protein was loaded on each lane. Antibodies were immobilized to Protein A Sepharose beads