Fig. 6

Substrate preferences of (a) FeDFR1a and (b) FeDFR2. Remaining substrates, (+)-dihydrokaempferol (DHK, left), (+)-dihydroquercetin (DHQ, middle) and (+)-dihydromyricetin (DHM, right), are plotted as a function of reaction time. Reaction mixtures contained the selected substrate (0.2 mg mL⁻¹), NADPH (2 mM), and either FeDFR1a or FeDFR2. Blue circles and orange triangles indicate experimental data obtained in the presence of 0.34 μM or 1.72 μM of DFR protein, respectively