Table 2 List of primer pairs used to isolate the α-type gliadins and in RT-PCR analysis
Primer name | Sequence | Reference |
|---|---|---|
L1 | 5’-ATGAAGACCTTTCTCATCCTTG | Xie et al. [35] |
R1 | 5’-TCAGTTRGTACCRAAGATGCC | |
RT a-gli F | 5’-AGACCTTTCTCATCCTTGCC | Li et al. [36] |
RT a-gli R | 5’-TGTACCAATGGAACTTGCTCT | |
G1Gli*1 | 5’-ACAGGTGAACCCATGCAAGAATTT | Piston et al. [37] |
G1Gli*2 | 5’-TGCATGATGATGGAATGTATGATGG | |
G2Gli*1 | 5’-TCATTCCCCCAACAACAACGG | Piston et al. [37] |
G2Gli*2 | 5’-AGGTTTGCATTGTTGCAAGAGGAT | |
G3 Gli*1 | 5’-GCAAATCCTGGTGCCACTGTCTCAA | Piston et al. [37] |
G3 Gli*2 | 5’-TGGGACATACACGTTGCACATGGTT | |
G4 Gli*1 | 5’-GATCCTGCGGCCACTATTTCAGCTC | Piston et al. [37] |
G4 Gli*2 | 5’-CAGGTGGCACATACACGTTGCACAT | |
QF18 | 5’-AAGGCAAGCAAGCAGTAG | Altenbach et al. [38] |
QR18 | 5’-GATTGTTGAGGTGATTGTAGC | |
QF21 | 5’-CAACCACCACAACAATTC | Altenbach et al. [38] |
QR21 | 5’-TTACATCTCTTCATTTCATAGG |