Table 1 The sequences of primers in the construction
The name of primer | The sequences of primer |
|---|---|
MdPIP1;3-F | 5’-GGATCCATGGAGGGCAAGGAAGAAG-3’ |
MdPIP1;3-R | 5’-TCTAGATTAGCTCCTGGACTTGAAAGG-3’ |
MdPIP1;3-GFP-F | 5’-GAATTCATGGAGGGCAAGGAAGAAG-3’ |
MdPIP1;3-GFP-R | 5’-GGATCCGCTCCTGGACTTGAAAGGAA-3’ |
MdPIP1;3-RT-F | 5’-TGATGCCAAGAGGAATGCC-3’ |
MdPIP1;3-RT-R | 5’-CACGCTTGGGATGACCACT-3’ |
MdActin-F | 5’-CAATGTGCCTGCCATGTATG-3’ |
MdActin-R | 5’-CCAGCAGCTTCCATTCCAAT-3’ |
SlActin-F | 5’-CCACGAGACCACATACAACT-3’ |
SlActin-R | 5’-TGAGGGAAGCCAAGATAGAG-3’ |
SlPIP1;2-RT-F | TTTCACTCACTAACTCCCATCAAT |
SlPIP1;2-RT-R | TAAAGAAAGAGGAAAGTAGCCACA |
SlPIP1;3-RT-F | ACCATCAAATAATCATCAGAGCA |
SlPIP1;3-RT-R | AGGATAAAATAAAAATTATTTTCAT |
SlPIP1;5-RT-F | ATGATTATGCCAAGGGAGATGA |
SlPIP1;5-RT-R | GCCAAATGAACCAAGAACACAG |
SlPIP2;1-RT-F | ACGTACCCGTGTTGGCACCTCTTCC |
SlPIP2;1-RT-R | ATGTTCGTCCCACGCCTTGTCACC |
SlPIP2;5-RT-F | GTCCTCTTCCAGCCATCCA |
SlPIP2;5-RT-R | ACCACTGAGCACAATGTTACCG |
SlPIP2;7-RT-F | ATTCCCATATCCCTGTGTTGGCTCC |
SlPIP2;7-RT-R | AGCTGCAGCTCTCAAAATGTATTGG |