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Fig. 1 | BMC Plant Biology

Fig. 1

From: Functional characterization of chitin-binding lectin from Solanum integrifolium containing anti-fungal and insecticidal activities

Fig. 1

Purification of eggplant lectin. a Ion exchange chromatography of the eggplant crude extract on a DEAE Sephadex A-25 column (1.0 cm × 20 cm). The column was pre-equilibrated with 0.02 M Tris-HCl buffer, pH 8. The bound protein was eluted using a 0.02–1 M NaCl gradient in 5 ml fractions at a flow rate of 0.5 ml/min and the fractions were monitored at 280 nm. A hemagglutination titer was assayed by red blood cells (RBCs) agglutination using trypsinized human blood cells. b Fractionation of peak I from the ion exchange column on a Sephadex G-75 column (2.6 cm × 40 cm) with 0.15 M NaCl in 0.02 M Tris-HCl (pH 8.0). The protein was eluted using the same buffer in 3 ml fractions at a flow rate of 0.5 ml/min. c Chitin gel column chromatography (1.0 cm × 20 cm) of the proteins eluted from Sephadex G-75 column. The bound protein was eluted using 20 mM acetic acid in 0.5 ml fractions at a flow rate of 0.1 ml/min

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