Fig. 7
From: Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress
Abiotic stress and hormonal treatments induced expression of CPK genes. qPCR analysis of the relative expression level of the CPK genes in cluster 4. Cotton seedlings were treated with 100 mM H2O2, 100 μM ABA, 1 mM SA, 100 μM MeJA, 200 μM ETH, 5 μM IAA, 0.5 μM GA, 20% PEG, 400 mM NaCl, 4 °C (cold), 37 °C (heat), wounding, or water (as a control), respectively. The GhUB7 gene was used as an internal control. The yellow, green and red bars represent mock, abiotic stresses and hormones treatments, respectively. The green and red arrows emphasize the significantly upregulated expression of target genes under salt and ETH treatments. The error bars indicate the standard deviation estimated from the four replicates (* P < 0.05, ** P < 0.01, t-test)