Fig. 8
From: Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress
Subcellular localization of GhCPKs. Nine GhCPKs (GhCPK8, GhCPK25, GhCPK38, GhCPK54, GhCPK55, GhCPK68, GhCPK88, GhCPK96, and GhCPK97) that were predicted to be involved in salt response were fused with YFP at the C-terminus and transiently expressed in 3-week-old tobacco leaf cells. YFP driven by the CaMV35S promoter was used as a control. Yellow fluorescence was observed 48 h post-Agrobacterium infiltration using confocal microscopy. The plasma membrane labeling by FM4–64 is shown as red fluorescence, the YFP observed in cell nuclei were marked with white arrows (scale bar = 10 μm)