Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities

Table 1 Starch synthase and AGPase activity in developing seeds (10–15 days after flowering) of double mutants, their parental lines, and wild-type

	Starch synthase			AGPase
Line	μmol min⁻¹ endosperm^{-1 a}			μmol min^− 1 endosperm^{-1, a}
Line	+ Citrate +Glycogen	+ Citrate -Glycogen	- Citrate + Glycogen	μmol min^− 1 endosperm^{-1, a}
SS1/ss2a^L/SS3a (Wild-type; Nipponbare)	11.09 ± 1.2 (100) ^b	2.22 ± 0 (100) ^c	1.17 ± 0 (100) ^d	0.259 ± 0.020 ^** (100) ^e
ss1 ^L /ss2a ^L /SS3a (i2–1)	5.28 ± 0.1 (48)	N.D. (0)	1.04 ± 0.2 (89)	0.245 ± 0.014^** (95)
SS1/ss2a ^L /ss3a (e1)	13.23 ± 0.5 (119)	5.36 ± 0.1 (241)	1.62 ± 0.1 (139)	0.324 ± 0.006^{, *} (125)
ss1 ^L /ss2a ^L /ss3a (#6002)	6.67 ± 0.6 (60)	N.D. (0)	0.76 ± 0.4 (73)	0.506 ± 0.031^* (196)

ND, Not detected
^aMean ± SE. n = 3 for all lines
^{b, c, d, e}Percentage of wild-type, Nipponbare
^*Significant differences between Nipponbare and mutant lines by the t-test at P < 0.05
^**Significant differences between double mutant line and the other lines by the t-test at P < 0.05

ISSN: 1471-2229