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Table 1 Starch synthase and AGPase activity in developing seeds (10–15 days after flowering) of double mutants, their parental lines, and wild-type

From: Analyses of starch biosynthetic protein complexes and starch properties from developing mutant rice seeds with minimal starch synthase activities

  Starch synthase AGPase
Line μmol min−1 endosperm-1 a μmol min− 1 endosperm-1, a
+ Citrate
+Glycogen
+ Citrate
-Glycogen
- Citrate
+ Glycogen
SS1/ss2aL/SS3a (Wild-type; Nipponbare) 11.09 ± 1.2
(100) b
2.22 ± 0
(100) c
1.17 ± 0
(100) d
0.259 ± 0.020 **
(100) e
ss1 L /ss2a L /SS3a
(i2–1)
5.28 ± 0.1
(48)
N.D.
(0)
1.04 ± 0.2
(89)
0.245 ± 0.014**
(95)
SS1/ss2a L /ss3a
(e1)
13.23 ± 0.5
(119)
5.36 ± 0.1
(241)
1.62 ± 0.1
(139)
0.324 ± 0.006*, **
(125)
ss1 L /ss2a L /ss3a
(#6002)
6.67 ± 0.6
(60)
N.D.
(0)
0.76 ± 0.4
(73)
0.506 ± 0.031*
(196)
  1. ND, Not detected
  2. aMean ± SE. n = 3 for all lines
  3. b, c, d, ePercentage of wild-type, Nipponbare
  4. *Significant differences between Nipponbare and mutant lines by the t-test at P < 0.05
  5. **Significant differences between double mutant line and the other lines by the t-test at P < 0.05