Fig. 6

Expression of GIS and TCL1. (a) Expression of GIS in seedlings of the Col-0 wild type, the 35S:TCL1 transgenic plant, and the tcl1, tcl1 try and tcl1 try cpc mutants. Total RNA was isolated from 10-day-old seedlings and qRT-PCR was used to examine the expression of GIS. ACT2 was used as an inner control, and the expression level GIS in the seedlings of the Col-0 wild type was set as 1. Data represent the mean ± SD of three replicates. *significantly different form that in seedlings of the Col-0 wild type, the 35S:TCL1 transgenic plant, and the tcl1 and tcl1 try mutants (P < 0.01). (b) Expression of GIS in protoplasts transfected with TCL1-VP and GL1GL3-VP. The plasmids of effector TCL1-VP or GL1GL3-VP were transfected into protoplasts isolated from rosette leaves of 3- to 4-week-old Col-0 wild type Arabidopsis plants. Contransfection of CAT was used a control. The transfected protoplasts were incubated at room temperature in darkness for 20–22 h, then total RNA was isolated and RT-PCR was used to examine the expression of GIS and GL2. The expression of ACT2 was used as a control. (c) Expression of TCL1 in the seedlings of the Col-0 wild type and the gis mutant. Total RNA was isolated from 10-day-old seedlings and qRT-PCR was used to examine the expression of TCL1. ACT2 was used as an inner control, and the expression level of TCL1 in seedlings of the Col-0 wild type was set as 1. Data represent the mean ± SD of three replicates