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Table 4 Summary of physiological properties and composition of B. napus cotyledons expressing AtGL3

From: The biochemical composition and transcriptome of cotyledons from Brassica napus lines expressing the AtGL3 transcription factor and exhibiting reduced flea beetle feeding

A. Insect behaviour and cotyledon biochemistry relative to Westar

Genotype

FB Feeding

Cotyledon Morphology

Cotyledon Orientation

Anthocyanin Accumulation

Cell Wall Carbohydrates

Cell Wall Lignin

AtGL3+a

Decreased

Abnormal

Vertical

Increased

No Difference

No Difference

K-5-8b

Decreased

Normal

Vertical

Decreased

No Difference

Decreased

B. Glucosinolate composition of transgenic lines relative to Westar

Genotype

Total GS

Progoitrinc

Glucoraphaninc

Gluconapinc

4-hydroxy-glucobrassicinc

Glucobrassicinc

4-methoxy-glucobrassicinc

AtGL3+

Decreased

No Diference

No Difference

Increased

No Difference

No Difference

Decreased

K-5-8

No Difference

No Difference

No Difference

Increased

Decreased

No Difference

Decreased

C. Glucosinolate composition of the transgenic lines relative to one another.

Genotype

Total GS

Progoitrinc

Glucoraphaninc

Gluconapinc

4-hydroxy-glucobrassicinc

Glucobrassicinc

4-methoxy-glucobrassicinc

AtGL3+

Decreased

Decreased

No Difference

Increased

No Difference

Increased

Decreased

K-5-8

-

-

-

-

-

-

-

  1. aAtGL3+, B. napus cv. Westar expressing AtGL3 under direction of CaMV 35S promoter
  2. bK-5-8, B. napus cv. Westar expressing AtGL3 under direction of CaMV 35S promoter as well as a TTG1 RNAi construct
  3. cProgoitrin, 2-hydroxy-3-butenyl-GS; Glucoraphanin, 4-methylsulfinylbutyl-GS; Gluconapin, 3-butenyl-GS; 4-hydroxyglucobrassicin, 4-hydroxy-3-indolylmethyl-GS; Glucobrassicin, 3-indolylmethyl-GS; 4-methoxyglucobrassicin, 4-methoxy-3-indolylmethyl-GS