Fig. 4
From: Purple Brassica oleracea var. capitata F. rubra is due to the loss of BoMYBL2–1 expression
Expression analysis of BoMYBL2–1 reporter constructs in transgenic Arabidopsis. Arabidopsis plants were transformed with reporter constructs containing GUS under the control of two different BoMYBL2–1 promoters: pMYBL2–1-w::GUS and pMYBL2–1v::GUS. a: Histochemical analysis of 10-day-old transgenic Arabidopsis seedlings. Seedlings were stained for 18 h at 37 °C using X-Gluc (Sigma–Aldrich, St. Louis, MO, USA) and washed in 95% ethanol. Non-transgenic plants (Col-0) and pCambia3301 were used as negative and positive controls for GUS expression, respectively. b: Expression analysis of GUS transcripts in transgenic and control plants. ACTIN (AtACT) was used as a normalization control