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Table 2 Differential metabolites of leaves between the budding stage and mid-flowering stage

From: Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages

Ionization mode

Metabolite no.

Adduct

RT(s)

m/z

Metabolite

VIP

Fold-change

P-value

ESI(+)

1

(M + H-H2O)+

458.83

120.08

Tyramine

2.65

1.47

0.0049

ESI(+)

2

(M + H)+

253.46

447.13

Sissotrin

1.48

3.91

0.0006

ESI(+)

3

(M + H)+

186.58

170.08

Pyridoxine

1.12

1.52

0.0006

ESI(+)

4

(M + H)+

489.09

220.12

Pantothenate

1.58

1.80

0.0000

ESI(+)

5

(M + H)+

190.21

431.13

Ononin

2.70

3.83

0.0196

ESI(+)

6

(M + H)+

190.05

269.08

Formononetin

1.52

3.69

0.0182

ESI(+)

7

(M + H)+

646.43

146.09

4-Guanidinobutyric acid

1.12

1.37

0.0000

ESI(+)

8

(M + H-H2O)+

285.03

313.27

1-Hexadecanoyl-sn-glycerol

1.41

1.50

0.0260

ESI(−)

9

(M-H)-

377.88

151.06

Xylitol

3.23

1.56

0.0001

ESI(−)

10

(M-H)-

62.82

137.02

Salicylic acid

3.66

2.29

0.0109

ESI(−)

11

(M-H)-

402.66

277.12

Pantetheine

1.19

2.47

0.0000

ESI(−)

12

(M + CH3COO)-

265.12

207.09

Mevalonic acid

2.00

1.88

0.0139

ESI(−)

13

(M-H)-

458.41

203.08

L-Tryptophan

2.33

1.74

0.0265

ESI(−)

14

(M-H)-

133.56

135.03

L-Threonate

1.06

1.30

0.0042

ESI(−)

15

(M-H)-

456.39

164.07

L-Phenylalanine

1.63

1.34

0.0143

ESI(−)

16

(M-H)-

134.08

89.02

Glyceraldehyde

1.32

1.23

0.0136

ESI(−)

17

(M-H)-

117.76

153.02

Gentisic acid

2.17

1.68

0.0364

ESI(−)

18

(M + CH3COO)-

539.79

181.07

D-Threitol

1.31

1.69

0.0001

ESI(−)

19

(M + CH3COO)-

486.17

209.07

D-Ribose

1.16

1.48

0.0001

ESI(−)

20

(M + CH3COO)-

465.33

223.08

D-Quinovose

3.74

2.29

0.0000

ESI(−)

21

(M-H)-

276.68

179.06

D-Mannose

1.77

1.58

0.0009

ESI(−)

22

(M-H)-

550.77

89.02

DL-lactate

2.00

1.19

0.0070

ESI(−)

23

(M + Na-2H)-

173.53

209.12

3-Hydroxycapric acid

1.72

1.56

0.0221

ESI(−)

24

(M + CH3COO)-

673.63

237.06

2-Dehydro-3-deoxy-D-gluconate

2.37

1.34

0.0030

ESI(+)

25

(M + H)+

696.52

246.18

Val-Lys

1.15

0.72

0.0362

ESI(+)

26

(M + H)+

656.61

274.19

Val-Arg

2.16

0.67

0.0061

ESI(+)

27

(M + CH3CN + H)+

79.12

261.15

trans-Zeatin

1.39

0.35

0.0487

ESI(+)

28

(M + H)+

105.57

123.06

Nicotinamide

7.09

0.61

0.0243

ESI(+)

29

(M + H)+

684.47

147.08

L-Glutamine

1.70

0.76

0.0159

ESI(+)

30

(M + H)+

730.92

148.06

L-Glutamic acid

2.26

0.79

0.0130

ESI(+)

31

(M + H)+

692.74

133.06

L-Asparagine

2.27

0.58

0.0017

ESI(+)

32

(M + H)+

613.65

288.20

Ile-Arg

2.19

0.66

0.0034

ESI(+)

33

(M + H)+

402.69

152.06

Guanine

2.20

0.56

0.0164

ESI(+)

34

(M + H)+

730.92

130.05

D-Pyroglutamic acid

1.95

0.81

0.0193

ESI(+)

35

(M + H)+

71.69

271.06

Apigenin

6.28

0.30

0.0265

ESI(+)

36

(M + H)+

677.16

87.04

4-Hydroxybutanoic acid lactone

2.90

0.81

0.0002

ESI(+)

37

(M + H)+

677.15

104.07

4-Aminobutyric acid

2.58

0.82

0.0004

ESI(+)

38

(M + H)+

338.63

496.34

1-Hexadecanoyl-sn-glycero-3-phosphocholine

2.17

0.68

0.0271

ESI(−)

39

(M-H)-

145.45

111.02

Uracil

1.77

0.49

0.0010

ESI(−)

40

(M-H)-

534.46

227.13

Traumatic Acid

2.23

0.63

0.0166

ESI(−)

41

(M-H)-

796.59

133.01

L-Malic acid

1.34

0.87

0.0327

ESI(−)

42

(M-H)-

730.88

146.05

L-Glutamate

2.28

0.83

0.0483

ESI(−)

43

(M-H)-

781.63

195.05

Galactonic acid

1.13

0.61

0.0423

ESI(−)

44

(M-H)-

277.29

134.05

Adenine

11.09

0.63

0.0010

ESI(−)

45

(M-H)-

688.71

113.04

Dihydrouracil

2.37

0.52

0.0021

ESI(−)

46

(M-H)-

595.57

163.04

4-Hydroxycinnamic acid

1.39

0.65

0.0077

ESI(−)

47

(M + Na-2H)-

113.11

237.15

3-Hydroxydodecanoic acid

1.44

0.48

0.0129

ESI(−)

48

(M-H2O-H)-

558.25

141.02

2-Oxoadipic acid

1.29

0.83

0.0063

ESI(−)

49

(M-H)-

202.24

133.05

2,3-Dihydroxy-3-methylbutyric acid

3.57

0.40

0.0014

  1. The fold-change (FC) was calculated as the average metabolite concentration in the leaves during the mid-flowering stage relative to the budding stage
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BMC Plant Biology

ISSN: 1471-2229

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