Fig. 5

Quantitative PCR analysis showing TaLr35PR5 expression in TcLr35 wheat plants inoculated with individual BSMV constructs and leaf rust pathogens, in wild-type (Mock) without BSMV and leaf rust inoculations, and in susceptible Thatcher wheat plant inoculated only with leaf rust pathogens. TaLr35PR5 gene expression levels are normalized to that of GAPDH. Values are means of triplicate reactions of three independent biological samples. Significant differences are represented by asterisks and error bars represent the standard deviation