Fig. 4
From: Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
Effects of pH and temperature on the activity of MpIspS. a pH: The reactions were performed in 50 mM sodium citrate (closed circle) or 50 mM MOPS buffer (opened circle) containing 50 μM DMAPP and 5 mM Mn2+ at 55 °C for 10 min. b Temperature: Reactions were performed in 50 mM MOPS buffer (pH 6.0) containing 50 μM DMAPP and 5 mM Mn2+ of the enzyme for 10 min. *Relative activity of 100% was 17.5271 U/mg