Fig. 3

Immunodetection of SOD enzymes in pollen protein extracts from 16 olive cultivars. a Total protein profiles of pollen extracts from 16 different olive cultivars after SDS-PAGE. The allergenic protein Ole e 1 is indicated with a bracket. Thirty micrograms of total protein were loaded per lane. Protein markers (kDa) are displayed on the left. b Western blot as in A probed with a commercial Ab to a chloroplastidic Cu,Zn-SOD enzyme (anti-CSD2) from Arabidopsis thaliana. The antibody was able to recognized a ~ 16 kDa chloroplastic SOD (CSD2) and a 23.4 kDa peroxisomal (CSD3) enzyme. A protein band of 15.2 kDa grouping cytosolic CSD1.1A, CSD1.2 and CSD1.3 forms, and a 14.5 kDa corresponding to the cytosolic CSD1.1B enzyme, respectively, were also visible. Arrows indicate monomeric enzymes, while arrowheads point out putative multimeric forms. 1, ‘Lechín de Sevilla’; 2, ‘Verdial de Vélez Málaga’; 3, ‘Hojiblanca’; 4, ‘Picudo’; 5, ‘Manzanilla de Sevilla’; 6, ‘Arbequina’; 7, ‘Cornicabra’; 8, ‘Blanqueta’; 9, ‘Loaime’; 10, ‘Lucio’; 11, ‘Bella de España’; 12, ‘Empeltre’; 13, ‘Farga’; 14, ‘Frantoio’; 15, ‘Galega’; 16, ‘Picual’