Fig. 7

Effect of reducing agents on stability of pollen recombinant SOD enzymes and alternative splicing model. a DTT-treated (+) and non-treated (−) of OeCSD1.1A (splicing A form; accession no. EU250770.1) and OeCSD1.1B (splicing B form; EU250769.1) recombinant SODs were electrophoresed and stained with CBB. Ten micrograms of protein were loaded per lane. Protein markers are displayed on the left. Asterisks indicate dimeric SOD forms. b Western blot as in A tested with an anti-olive Cu,Zn-SOD Ab. Asterisks show SOD dimers. c In-gel SOD activity of DTT-treated (+DTT) and non-treated (−DTT) rOeCSD1.1A proteins. Asterisk indicates an rOeCSD1.1A dimer. d Schematic representation (exons and introns) of olive pollen OeCSD1.1A and OeCSD1.1B genes. Green arrowheads point out the position and length of the introns in the cDNA. A total of six introns were predicted by using the bioinformatics tool provided by Cruz et al. (2016). The position and length of the seven exons, as well as the alternative splicing of exon 3, are also indicated. The 3D models of both the OeCSD1.1A and OeCSD1.1B forms of the enzyme are shown. The missing fragment in the OeCSD1.1B form is indicated with an orange circle. e Detail of the nucleotide sequence at the alternative splicing zone in the OeCSD1.1A and OeCSD1.1B transcripts