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Table 1 Mean yield in cotyledonary somatic embryos (SEs) of Douglas-fir from 1ry and 2ry embryogenic lines

From: Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Line

Mean no. of SEs g− 1 f.w.

Primary

 TD17

30

± 19a

 SD4

478

± 139c

Secondary

 TD17–1

241

± 70b

 SD4–2

3131

± 34e

 SD4–6

1515

± 297d

 SD4–8

1821

± 363d

  1. Values are means of 3 biological and 5–6 technical replicates ±95% confidence limits. Significant differences (p < 0.05) in multiple comparisons of means are indicated by different letters