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Fig. 3 | BMC Plant Biology

Fig. 3

From: TM8 represses developmental timing in Nicotiana benthamiana and has functionally diversified in angiosperms

Fig. 3

Effects of silencing and overexpression of NbTM8 and NbSVP. a Extra floral organs in NbTM8- and NbSVP-VIGS plants, similar to the 35S:miR172 phenotype. b Twisted petal tubes in some NbTM8-VIGS plants. c Split petal tubes in NbTM8-VIGS and 35S:miR172 plants. d NbTM8-VIGS plants have smaller, greenisher and twisted leaves in the inflorescence. e Early flowering and accelerated development in NbTM8-VIGS and NbSVP-VIGS plants. Asterisks indicate significant differences at the 0.01 significance level applying a Student’s t-test. f NbSVP-VIGS plants are early flowering compared to control plants. (Arrows indicate first flowers). g Relative expression of NbTM8, miR172 and NbSVP in inflorescence tissue of NbTM8-VIGS and control plants. h Relative expression of NbSVP, miR172 and NbTM8 in NbSVP-VIGS and control plants. i Strongest 35S:NbTM8 line grows more slowly and only develops juvenile leaves. j Sepal and bracts of EV compared to sepal and sepaloid bracts of 35S:NbTM8. k Close-up of adult leaf compared to 35S:NbTM8 leaf. l Comparison of chlorophyll levels between 4 month old EV plants and 8 month old 35S:NbTM8 plants. m Reduced petal tubes in 35S:NbTM8, 35S:NbSVP, and 35S:NbAP2 flowers. N) Strongly reduced petal tube of 35S:NbTM8 flower. o Petaloid sepals in 35S:NbSVP lines. p Leaf-like sepals in 35S:NbSVP lines in comparison to EV. q Strongly reduced petal tube and enlarged sepals of 35S:NbSVP flower. r Leaf like carpels in 35S:NbSVP flower. s Scanning electron microscopy of EV sepals and petals compared to 35S:NbTM8 and 35S:NbSVP petals. Scale bar represents 50 μm. t Relative expression levels of NbTM8 and miR172 in inflorescence tissue of control and 35S:NbTM8 lines. u qPCR of NbSVP and miR172 in inflorescence tissue of control and 35S:NbSVP. EV: empty vector control lines. Error bars are standard error of the mean of three technical replicates

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