Skip to main content
Fig. 2 | BMC Plant Biology

Fig. 2

From: The antibiotic peptaibol alamethicin from Trichoderma permeabilises Arabidopsis root apical meristem and epidermis but is antagonised by cellulase-induced resistance to alamethicin

Fig. 2

Alamethicin mainly permeabilises epidermal cells of A. thaliana seedlings. Seedlings were grown for 5 days in ½ MS. Confocal laser scanning microscopy images were collected as slices through the root in different 2-D planes. a, b, e Control plants, c, d Cellulase-treated plants. After treatment with cellulase in mannitol or in mannitol only (control) for 3 h, they were incubated for 15 min with 20 μg ml− 1 alamethicin in mannitol, where PrI was included the last 5 min. PrI can be seen to stain nuclei and the cytoplasm, whereas cell walls are not stained. Alam, alamethicin; Fluor, fluorescence; BF, bright field; Sum Z projection are the fused Z slices and reflects a compressed 3-D image; Z-X slices are selected images along the y-axis, where the levels analysed are indicated by dotted lines; X-Y slices are selected images along the Z-axis at different depths in the root; rc, root cap; am, apical meristem zone; bm, basal meristem zone; eez, early extension zone; ez, extension zone; atr, atrichoblast; tr, trichoblast. One of three experiments with similar results is shown. Bar corresponds to 25 μm

Back to article page