Fig. 2
From: Cytosolic GAPDH as a redox-dependent regulator of energy metabolism
Redox-dependent binding of GapC1 to VDAC3. a Association and dissociation behavior of reduced and oxidized recombinant GapC1 added to bilayer-incorporated recombinant VDAC3 at the indicated concentrations is shown as original traces. For reduction, samples were pre-treated with 2 mM DTT, for oxidation with 0.5 mM H2O2 + 0.5 mM GSH. b Equilibrium dissociation constants (KD) of pre-treated GapC1 binding to VDAC3 were obtained as described in Material & Methods. Error bars represent SD, p-value by Students t-test p ≤ 0.005