Fig. 5
From: Cytosolic GAPDH as a redox-dependent regulator of energy metabolism
Redox-dependent localization of GapC and Trx-h3. Vectors encoding (a) GapC1- or (b) GapC2-fusion constructs of YFPNE, and Trx-h3-YFPCE were co-transformed into mesophyll protoplasts. The fluorescent signals from the YFP complementation are shown in cyan. The nuclei are shown in magenta as labeled by bZip63 fused to mCherry. Colocalization events from the complemented YFP and the nuclear-located bZip63-mCherry proteins are shown in white. Transformed protoplasts were treated with 0.2 mM H2O2 or 10 mM DTT for 30 min. Scale bars were calibrated to 10 μm