Fig. 3

Pattern of GsMATE expression in response to acidic aluminum exposure. a Pattern of GsMATE expression under acidic aluminum exposure. b Temporal expression pattern of GsMATE under acidic aluminum exposure. Two days after germination, the seedlings were transferred to solutions of 0, 25, 50, 75, and 100 μM AlCl₃ (pH 4.3, 0.5 mM CaCl₂). After 6 h, root tip samples (6 cm long) were obtained from the seedlings for the analysis of the GsMATE expression patterns. To analyze the temporal expression pattern of GsMATE, seedlings (2 days after germination) were cultured in a solution of 0.5 mM CaCl₂ (pH 4.3) for 24 h, and then transferred to the solution of 50 μM AlCl₃ (pH 4.3, 0.5 mM CaCl₂). Root tip samples (6 cm long) were obtained from the seedlings after the treatments of 2, 4, 6, 8, 12, and 24 h. Data were represented as the mean ± SE of three biological replicates; the same letter on each column set indicates no significant difference and different letters indicate a statistically significant difference according to analysis of variance (t-test, p = 0.05). BW69, JW81: two lines of Glycine soja. Data were analyzed using SPSS20, and plotted using EXCEL2000