Fig. 2
From: Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity
Over-expression of ERF8 triggers programmed cell death (PCD). (a) DEX-inducible overexpression of ERF8 triggered cell death in transgenic Arabidopsis at 48 h after DEX treatment. Scale bar = 1 cm (b) DEX-inducible expression of ERF8 in Col-0 and sid2–1 background led to PCD in Arabidopsis. Leaves from Col-0 and various DEX-inducible ERF8-OE lines were sprayed with 30 μM DEX and stained with trypan blue solution. Images were taken 2 days after DEX treatment. Scale bar = 1 mm. (c) Wildtype ERF8 but not ERF8L176A, L178A elicited strong cell death in N. benthamiana. Photo taken 6 days post inoculation (dpi) (+/ red circle = cell death, −/ white circle = no cell death). (d) ERF8 overexpression induced cell death in N. benthamiana at both 25 °C and 28 °C 3 dpi. (e), Transient expression of YFP-tagged ERF8 in N. benthamiana localizes to the nuclei (example nucleus labeled N). Scale bar top = 100 μm, bottom = 50 μm