Fig. 4

Impact of ANN5 expression on seed yield. (a) Dry seeds isolated from siliques at positions 36–40 of the main bolt and embryos dissected from rehydrated seeds of wild-type Arabidopsis (Col-0), ANN5-RNAi_15, ANN5-RNAi_13, and ANN5-OE_2. Scale bars = 500 μm. (b) Average sizes of pooled seeds from a single biological replicate. n = 1000. Three independent experiments were performed with similar outcomes. Asterisks indicate significant differences compared with wild-type seeds (one-way ANOVA, Dunnett post hoc test, *p < 0.05; **p < 0.01; ***p < 0.001). Bars represent SD. (c) Average sizes of seeds collected from siliques at specified positions on the main bolt, pooled from a single biological replicate. n = 120–150. Bars represent SD