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Fig. 3 | BMC Plant Biology

Fig. 3

From: The sucrose non-fermenting-1-related protein kinases SAPK1 and SAPK2 function collaboratively as positive regulators of salt stress tolerance in rice

Fig. 3

The detection of sapk1, sapk2 and sapk1/2 mutants and overexpression plants of SAPK1 and SAPK2. (a) Diagram showing that sgRNAs targets of SAPK1 and SAPK2 were used in the CRISPR/Cas9 system to generate sapk1, sapk2 and sapk1/2 mutants. Two independent mutant lines of sapk1, sapk2 and sapk1/2 with different deletions were obtained. (b) Ser and Thr residues in the Activation Loop are essential for the activity and/or activation of SAPK1 and SAPK2 in Response to Hyperosmotic Stress. (c). Accumulation of SAPK1 and SAPK2 transcripts in SAPK1-OE lines, SAPK2-OE lines and wide type plants. Transcript accumulation was assessed by qRT-PCR. Error bars indicate the SD (n = 3). ** indicate statistically significant differences between mutant lines and wild type plants (P < 0.01)

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