Skip to main content

Table 2 Characterisation of DFR from enzyme preparations of cv. ‘Christmas Feeling’ bracts

From: The rare orange-red colored Euphorbia pulcherrima cultivar ‘Harvest Orange’ shows a nonsense mutation in a flavonoid 3’-hydroxylase allele expressed in the bracts

Substrate

DHK

DHQ

DHM

pH optimum

6.50

6.25

5.75

Temperature optimum

40 °C

40 °C

40 °C

Time linearity [min]

20

20

20

Protein linearity [μg in assay]

2.6

0.2

0.2

Apparent kcat [μmol/kg*s]

97

78

3105

Apparent Km [μM]

14

0.9

29

kcat/Km [l/s*kg]

7

86

107