Table 2 Degree of similarity of individual genome guides to homeologous genes in wheat

pRMH110

All genomes

gRNA

TTGTTTGCCAAGATTTTCCA

A

TTGTTTGCCAAGATTTTCCA

n/a

B

TTGTTTGCCAAGATTTTCCA

n/a

D

TTGTTTGCCAAGATTTTCCA

n/a

Barley

TTGTTTGCCAAGATTTTCCA

n/a

pRMH120 and pRMH123

A genome

gRNA

CTGTGTATGAAGTTGTCCGG

A

CTGTGTATGAAGTTGTCCGG

n/a

B

CTGCGTATGAAGTTGTCCAG

2

D

CTGTGTATGAAGTTGCCCAG

2

pRMH121

B genome

gRNA

CTGCGTATGAAGTTGTCCAGC

A

CTGTGTATGAAGTTGTCCGGC

2

B

CTGCGTATGAAGTTGTCCAGC

n/a

D

CTGTGTATGAAGTTGCCCAGC

2

pRMH125

D genome

gRNA

GCCAGGGGAAGTCGAACTAA

A

GCCAAGGGA––––––––––––

11

B

GCCAAGGGA––––––––––––

11

D

GCCAGGGGAAGTCGAACTAA

n/a