Fig. 2

Immunoprecipitation and Western blotting of TtRec8 in LDN. Top: M-protein size marker; 1-total protein extracted from the anthers primarily at early prophase I; 2-proteins immunoprecipitated by anti-TtRec8 antibody; 3-negative control without TtRec8 and other proteins (Note: this sample was prepared from the immunoprecipitation experiment without anti-TtRec8 antibody added in the immunoprecipitation reaction); and 4-supernatant from the immunoprecipitation experiment with anti-TtRec8 antibody added in the reaction. Bottom: M-protein size marker; 1-interphase; 2-early prophase I; 3-metaphase I/anaphase I; and 4-metaphase II/anaphase II