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Fig. 3 | BMC Plant Biology

Fig. 3

From: General control non-repressible 20 (GCN20) functions in root growth by modulating DNA damage repair in Arabidopsis

Fig. 3

The mutation in GCN20 results in cell cycle arrest at G2/M phase. a Analysis of cellular DNA replication in wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 plants by an EdU incorporation assay. b Fluorescence nuclei number of wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 root meristems in EdU incorporation assay. c-f The expression of CDKB1;1 (c), KRP2 (b), WEE1 (e) and CDKB2;1 (f) were assayed by qPCR in the wild type, gcn20–1 and GCN20::GCN20 gcn20–1. The expression level of the wild type is set to 1 and ACT2 was used as an internal control. Data shown are means ± SEM. Asterisks indicate significant differences with respect to each control (Student’s t test): ***, P < 0.001

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