Fig. 2

Mutation in OsPHR3 affects vegetative and reproductive traits under different N and NO₃⁻ regimes. Seeds of the WT and OsPHR3 mutants (osphr3–1, osphr3–2 and osphr3–3) were grown hydroponically in IRRI solution for 2 weeks. Seedlings were then transferred to (a, b) 2.5 mM N and 0.25 mM N and (c, d) H NO₃ and L NO₃⁻ media for 7 d. e, f WT and the mutants were also grown in a pot soil for 17 weeks (grain-filling stage). Phenotypes of the (a) seedlings, (c) root system architecture and (e) panicles were observed. Data are presented for (b) biomass, (d) average length of adventitious roots, (f )per cent seed-set and (g) grain yield/plant. Values in (b, d and e) are means ±SE (n = 5) and different letters on the histograms indicate that the values differ significantly (P < 0.05, one-way ANOVA)