Fig. 6

Mutation in OsPHR3 affects the expression of NO₃⁻ and NH₄⁺ transporter and nitrate reductase genes. Seeds of the WT and mutants (osphr3–1 and 3–2) were grown hydroponically in IRRI solution for 2 weeks, deprived of N for 3 d and then transferred to 2.5 mM N and 0.25 mM N media for 1 d. Roots were harvested and qRT-PCR was employed for determining the relative expression levels of genes encoding NO₃⁻ (OsNRT1.1a, OsNRT2.3a and OsNRT2.4), NH₄⁺ (OsAMT1.1, OsAMT1.2 and OsAMT1.3) transporters and nitrate reductase (OsNia1 and OsNia2). Actin was used as an internal control and + N WT values were normalized to 1. Values are means ±SE (n = 3) and different letters on the histograms indicate that the values differ significantly (P < 0.05, one-way ANOVA)