Fig. 4

Phenotypes of 35S::PvCO1 or 35S::PvCO2 transgenic plants. a Flowering phenotypes of wild type and 35S::PvCO1 lines. b Analysis of number of rosette leaves of different genotype plants at blooming. c Real-time quantitative RT-PCR analysis of transcript accumulations of PvCO in different transgenic lines. The total RNAs were isolated from 14 d seedlings at ZT 4 h under LD.Y axis, relative transcript level of PvCO1 or PvCO2 compared with that of AtActin. Error bar indicates standard deviation. a, b indicate highly significant differences. At least 15 plants per line from the T3 generation were grown to analyze the flowering time phenotype