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Fig. 2 | BMC Plant Biology

Fig. 2

From: Modulation of auxin and cytokinin responses by early steps of the phenylpropanoid pathway

Fig. 2

KMD1/KFB20 targets PAL and not ARR1 for proteasomal degradation. a Rosettes of 14-day-old plants are shown above the representative immunoblots to underline the correlations of rosette size and protein accumulation level. Rosettes of two independent 35S::KMD1/KFB20 (OE) lines are shown. The kfb tr. refers to the kfb20–1 kfb1–1 kfb50–1 triple mutant. LSU, large subunit of RuBisCO is a loading control. b GUS activity in 4-day-old seedlings treated with 25 nM benzyladenine (BA) for 4 h prior to GUS staining. Two seedlings per line are shown. c Anthocyanin accumulation in 12-day-old plants is presented as the absolute absorbance of the methanolic extract at 520 nm (A520) per ten plants. Data are shown as mean ± SD (n ≥ 3). The significance of the difference of anthocyanin levels between Col-0 and the kfb triple mutant and between Col-0 and OE#1 for each treatment is noted (*, P < 0.05; **, P < 0.01; ***. P < 0.001; two-way ANOVA with Bonferroni’s multiple comparisons test). Insert illustrates the accumulation of anthocyanins in representative rosette leaves of 60-day-old plants. d Effect of t-CA and p-coumaric acid (CuA) on the growth of OE#1 and the arr1–3 arr10–1 arr12–1 triple (arr tr.) mutant. Plants were grown on MS/2 media containing the denoted doses of t-CA and CuA for 18 days. e Statistical analyses of the effect of t-CA and p-coumaric acid (CuA) on the fresh weight (FW) of plants shown in d. Data are presented as mean ± SD (n ≥ 12 pools of 8 plants).). The significance of the difference between the control and the treated samples is noted for each line (****, P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparisons test)

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