Fig. 3

Analysis of selected BY-2 calli supertransformed with pER8-IR-GFP. RT-qPCR analysis of IR-GFP transcript levels in five randomly selected spontaneously silenced calli (marked from − 1 to − 5) and five non-silenced calli (marked from + 1 to + 5) and one additional non-silenced callus grown on the induction medium with β-estradiol (marked as “e”) and on the control medium with DMSO (marked as “d”). Each callus represented independent transformation event. a Fluorescence of analyzed calli (in arbitrary units), signals below 4000 represent the background; b the level of the sense GFP transcript (RT: oligo dT primer, qPCR: F1 and R1 primers); c the level of transcripts with the sense intron of IR-GFP (representing both the unspliced transcript and the spliced intron; RT: R5 primer, qPCR: F5 and R5 primers); d the level of transcripts with the antisense intron of IR-GFP (RT: F5 primer, qPCR: F5 and R5 primers); e the level of the sense IR-GFP unspliced transcript (RT: R5 primer, qPCR: F4 and R5 primers); f the level of the antisense IR-GFP unspliced transcript (RT: F5 primer, qPCR: F5 and R4 primers); g the level of the sense spliced transcript (RT: R6 primer, qPCR: F6 and R6 primers)