Fig. 6

Read-throught transcripts of the pER8 T-DNA in selected BY-2 calli transformed with pER8-GFP. Semiquantitative RT-PCR analysis of transcript levels in five independent calli untreated with β-estradiol (the same calli as in Fig. 5). cDNA was prepared using R1 primer. a The level of the EF1α transcript (internal standard); b the level of read-through (r.-t.) pER8 T-DNA transcript (F7 and R2 primers); c amplification of RNA samples that were not treated with reverse transcriptase to ensure that there was no DNA contamination