Table 4 Primers for real-time quantitative PCR
From: Iron and callose homeostatic regulation in rice roots under low phosphorus
Gene Symbol | Sense primer (5′-3′) | Reverse primer (5′-3′) | Product length | Tm |
|---|---|---|---|---|
OS07G0258400 | TTTGGGTGATTTTGATTGGC | CTTCTGGAATATCGGAAGCA | 180 | 55.00 54.94 |
OS05G0472400 | TTTCTTGCTCTAAGCAGTGT | CCACAAAAAGTCTACACCCA | 169 | 54.91 55.49 |
OS04G0542200 | CAAGACGGGACATCTAACAT | AGGCACTGTAGAACAAGAAG | 116 | 54.87 55.01 |
OS03G0843100 | ATTGGATTGCTTGAGGTGAT | GAAGCGGCTGTACTATGTTA | 130 | 54.97 55.00 |
OS03G0307200 | TGAGTGCGTGCATAGTAATC | TCATCCACACAACAAGAACA | 122 | 55.67 55.12 |
OS02G0306401 | GTTTGCCTTTTATGGCCTTT | CACTATATATGGCTCGCCTC | 105 | 54.99 55.01 |
OS02G0650300 | GAAAGCAGCATGACAAGTTT | AAAAACGACTGCAAAAGGAG | 127 | 55.08 55.02 |
OS02G0649900 | TCCTTAACTTGCTTCCACTC | GGAAGAAGCTCCATAAGAGG | 183 | 54.99 54.93 |
OS04G0538400 | AATAATCAAGGGGTTGTGCT | AACCATTACACTTACACCCC | 142 | 54.88 54.94 |