Fig. 8

Evaluation of disease resistance and expression of defense signaling-related genes in spl21 and WT. a Reactions to Xoo race PXO99. 1–2: WT before inoculation; 3–4: spl21 before inoculation; 5–7: WT after inoculation; 8–10: spl21 after inoculation; 11–16: complementation lines. b Mean lesion length/leaf length radio after inoculation of plant leaves with PXO99. Data represent the lesion length means ± SD from 5 independent plants at the tillering stage (Student’s t-test: **, P ≤ 0.01). c Relative expression of seven defense signaling-related genes in WT and spl21 at the tillering stages analyzed by qRT-PCR. The expression level of each gene in WT was normalized to 1; data represent the mean ± SD of three biological replicates (Student’s t-test: **, P ≤ 0.01)