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Fig. 5 | BMC Plant Biology

Fig. 5

From: An invaluable transgenic blueberry for studying chilling-induced flowering in woody plants

Fig. 5

Transgene insertion position in Mu-Legacy and its effect on expression of adjacent gene(s). a, Southern blot analysis of Mu-Legacy (M) and Legacy (L). About 30 micrograms DNA was digested using HindIII. The gusA coding region was used as the probe. b, The T-DNA was inserted into a blueberry retro-transposon region, which locates at the upstream of VcRR2. c92529_g4_i1, c92529_g4_i2, c96767_g2_i12 are the transcripts near the right repeat of the T-DNA insertion region. *LB T-DNA repeat: there was a deletion of 18 from 5′-end. *RB T-DNA repeat: this repeat (25 bp) and 37 bp up its 5′-end, including 16 bp belonging to the NOS terminator, were deleted. The insertion increased overexpression of the VcRR2 in gene contigs c92529_g4 and c96767_g2 in both leaf and flower bud tissues of Mu-Legacy and also in leaf tissues of Mu-Legacy-T1 (Table 1)

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