Fig. 2From: Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheatGene sequences of targeted mutagenesis in wheat protoplasts. Detection of mutations in (a) Pinb-gR1, (b) Pinb-gR2, (c) Pinb-gR3, (d) waxy-gR1, (e) waxy-gR2, (f) waxy-gR3, and (g) DA1-gR1. The wild-type sequences of the target genes are shown with the PAM sequences underlined in black. Inserted and substituted nucleotides are shown in red. Deletions of nucleotides are shown as dotsBack to article page