Table 1 Illumina sequencing results of targeted mutations in wheat protoplast
From: Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat
Target Name | Clean reads | Mapped information | Target editing information | ||||||
|---|---|---|---|---|---|---|---|---|---|
Mapped | % | SNP | % | indel | % | Total | % | ||
reads | |||||||||
DA1gR1 | 462,296 | 456,040 | 99.91 | 30,793 | 6.75 | 242 | 0.05 | 31,035 | 6.81 |
PinbgR12-g1 | 1,361,836 | 1,357,466 | 99.68 | 60,923 | 4.49 | 1094 | 0.08 | 62,017 | 4.57 |
PinbgR12-g2 | 1,361,836 | 1,357,466 | 99.68 | 54,913 | 4.05 | 4455 | 0.33 | 59,368 | 4.37 |
PinbgR3 | 596,786 | 573,600 | 96.11 | 21,982 | 3.83 | 178 | 0.03 | 22,160 | 3.86 |
waxygR12-g1 | 1,235,538 | 1,151,644 | 93.21 | 20,080 | 1.74 | 14 | 0 | 20,094 | 1.74 |
waxygR12-g2 | 250,826 | 164,766 | 65.69 | 4208 | 2.55 | 6 | 0 | 4214 | 2.56 |
waxygR3 | 121,510 | 120,336 | 99.03 | 2819 | 2.34 | 79 | 0.07 | 2898 | 2.41 |